Expression Of MLC20 And Related Molecules In Rats With Total Parenteral Nutrition

Objective:Total parenteral nutrition(TPN)is a method of infusion of nutrient solution through intravenous or peripheral venous cannulation,usually used in the can not or can not fully from the gastrointestinal tract to absorb nutrients,and the use of this method Can make the patient's body protein,calories,electrolytes,vitamins,etc.are added,so as to achieve a more satisfactory results.TPN has been widely used in clinical treatment,in improving the tolerance of critically ill patients,as well as the success rate of rescue,improve the therapeutic effect,promote rehabilitation and so has very important significance.Although TPN as a clinical treatment of patients with life-sustaining means of intervention,long-term can not be carried out in patients with enteral nutrition support is of great significance,but the risk caused by the still can not be ignored.TPN can cause intestinal epithelial cell apoptosis,inhibition of proliferation,resulting in decreased intestinal smooth muscle contract,Therefore,in-depth exploration and study of TPN function of intestinal smooth muscle contractility when the relevant mechanisms to help the majority of medical practitioners in the clinical application of the process to prevent the occurrence of TPN-related complications.?-3 PUFAs as a specific immune nutrients,for the synthesis of ester media,cytokine release,leukocyte and endothelial cell activation has a very important regulatory role,but also in the regulation of the body in the case of trauma and infection,excessive inflammatory response process play an important role.However,whether the fatty acid of fish oil has an effect on the intestinal smooth muscle contract function has not been known,and no reports have been reported at home or abroad.In the present study,the expression of MLC20?p-MLC20,MYPT1?p-MYPT1,CPI-17? p-CPI-17 proteins in the intestine after TPN were detected by western blot,and the mechanisms involved in intestinal mucosal barrier were studied.?-3 fish oil fat emulsion to intervene in order to observe its role in improving the intestinal mucosal barrier.In order to provide a laboratory basis for clinical prevention and treatment of TPN-related complications.Material and methods: Eighteen SPF male Sprague Dawley rats were randomly divided into three groups: normal saline group,TPN group and fish oil group.Commercial feeding of mouse food and water to maintain light and dark the 12-hour cycle,the temperature maintained at 22 ± 2 ?,relative humidity 55 ± 5%,ventilation 15 times / h.After successful anesthesia in each group,sterile environment by the right jugular vein into the silicone tube after eating water for two days to facilitate recovery from surgical stress.The rats in the TPN group were given TPN and TPN in the fish oil group were replaced with omega-3 fish oil and fat emulsion in the TPN group.The rats in the TPN group were fed with the same dose of saline for 3 weeks.Liquid,TPN group and fish oil group during fasting without water during infusion.The infusion volume of each group was 20 m L on day 1,40 m L on day 2 and 60 m L on day 3 to day 7.After treatment,all rats were killed by cervical dislocation fast to take the small intestine proximal 1/3,middle 1/3 and distal 1/3 tissue,using Western blot detection of small intestinal tissue MLC20,p-MLC20,MYPT1?p-MYPT1,P-CPI17,CPI-17,protein expression levels.Results:The expression of MLC20,p-MLC20,MYPT1,p-MYPT1,CPI-17,p-CPI-17 protein in the proximal small intestine of TPN group and fish oil group did not change significantly compared with the saline group(P 0.05).The expression of MLC20,p-MLC20,MYPT1?p-MYPT1,CPI17,p-CPI17 protein in the small intestine of TPN group and fish oil group was not significantly different from that of the saline group(P> 0.05).The expression of MLC20,MYPT1,CPI17 protein in distal end small intestine of TPN group were not significantly different from that of saline group(P >0.05).Compared with TPN group,(P <0.05).The expression of p-MLC20,p-MYPT1,p-CPI17 in the distal small intestine of TPN group was significantly lower than that of saline group(P <0.05).Compared with TPN group,p-MLC20,p-MYPT1,p-CPI17 protein were significantly increased(P <0.05).Conclusion:1.TPN can inhibit the phosphorylation of MLC20,MYPT1 and CPI-17 protein,and decrease the intestinal smooth muscle contractility,2.The effect of fish oil on the contraction of intestinal smooth muscle was observed.