The Clinical Characteristics And Prognostic Significance Of AID Gene,miR-181b,and MiR-155 Expression In Acute Leukemia

First part: Expression of activation-induced cytidine deaminase gene inleukemiaObjective: This study aimed to investigate the characteristics of activation-induced cytidine deaminase(AID)gene expression level in de novo acute leukemia(AL)patients,chronic myeloid leukemia chronic phase(CML-CP),chronic myeloid leukemia blastic crisis(CML-BC)patients and leukemia cell lines.Methods: The expression level of AID mRNA was measured in 89 cases of newly-diagnosed acute lymphoblastic leukemia(ALL)patients,79 cases of de novo acute myeloid leukemia(AML)patients,5 cases of CML-BC patients,5 cases of CML-CP patients and leukemia cell lines NB4,THP-1,KG-1,Raji,K562 by real-time quantitative reverse transcriptase polymerase chain reaction(RT-PCR),and bone marrow mononuclear cells of 16 normal healthy donors were used as the control group.Results: The expression levels of AID mRNA in 89 cases of ALL and 79 cases of AML were 0.006~7463.175 and 0.005~69.107,the median expression levels were 3.785 and 1.812,the expression level of AID mRNA in the normal control group was 0.146~4.707,and the median expression level was 1.483,respectively.The AID expression levels of ALL,B-ALL,Burkitt leukemia,M4 patients and Raji cells were significantly higher than those of the normal control group(all P<0.05).Nevertheless,the AID mRNA expression levels of M3 patients and NB4,KG-1 cells were lower than those of the normal control group(all P<0.05).Furthermore,the AID mRNA expression levels of K562 cell were strikingly higher than that of the CML-CP patients(P=0.025),so were those of CML-BC,chronic myeloid leukemia myeloid blast crisis(CML-MBC),chronic myeloid leukemia lymphoblastic blast crisis(CML-LBC)patients.Conclusion: AID gene shows high expression level in B-ALL,Burkitt leukemia and M4,low expression level in M3 and KG-1 cells,and obvious high expression level in CML-BC.Second part: The clinical characteristics and prognostic significance of AID gene,mi R-181 b,and mi R-155 expression in adult patients with de novo B-cell acute lymphoblastic leukemiaObjective: This study aimed to investigate clinical characteristics and prognostic significance of activation-induced cytidine deaminase(AID)gene,mi R-181 b and mi R-155 expression in de novo adult B-cell acute lymphoblastic leukemia(B-ALL)patients.Methods: The expression level of AID gene,mi R-181 b and mi R-155 in bone marrow mononuclear cells was measured in 76 cases of newly diagnosed B-ALL patients and 16 cases of normal healthy donors by real-time quantitative reverse transcriptase polymerase chain reaction(RT-PCR).Results: AID gene and mi R-155 expression levels were higher in B-ALL patients than in healthy controls,while mi R-181 b expression level was lower in B-ALL patients.Ph+ B-ALLs had higher AID expression level than Ph— B-ALLs,and its high expression was associated with BCR-ABL.In addition,B-ALL patients with AIDhigh or mi R-181 blow expression had a shorter overall survival(OS).AIDhigh with mi R-181 blow,AIDhigh with mi R-155 low,mi R-181 blow with mi R-155 low,AIDhigh with mi R-181 blow and mi R-155 low expression were associated with shorter OS.Combination of the three molecules are more accurate predictors for unfavorable OS compared with univariate group.Conclusion: AID,mi R-181 b and mi R-155 provide clinical prognosis of adult de novo B-ALL patients and may refine their molecular risk classification.