| BACKGROUND:Spinal cord injury(SCI)is a disease causing a variety of motor and sensory dysfunctions,abnormal muscle tone and pathological reflex.Clinically,human umbilical cord mesenchymal stem cell transplantation has become a therapy for SCI.OBJECTIVE:To investigate the effect of local transplantation of human umbilical cord mesenchymal stem cells in different time after spinal cord injury in rats.METHODS:100 SPF male adult Sprague-Dawley rats were randomly divided into five groups:blank control group,SCI group,post-model 3-,7-,21-day transplantation groups(n=20 per group).Animal models of T10 SCI were made by Allen’s method in the latter four groups,and rats in the three transplantation groups were given HUCMSCs transplantation at 3,7,21 days after SCI,respectively.RESULTS AND CONCLUSION:Compared with the SCI group,improved motor function scores,decreased interleukin-2 level,and increased serum interleukin-10 level were observed in the three transplantation groups at 49 days after modeling,indicating SCI was improved significantly in these three transplantation groups,especially in the post-model 7-day transplantation group.These findings suggest that human umbilical cord mesenchymal stem cell transplantation for SCI repair improves the movement function of rats,and cell transplantation at 7 days after modeling has achieved best outcomes. |
