Study On The Anti-myocardial Ischemia Effects Of Shenqi Fuzheng Using Network Pharmacology Approach

Shenqi Fuzheng Injection(SQ)is composed of two Chinese traditional medicines Codonopsis pilosula(DS)and Astragalu(HQ).It is mainly used for the adjuvant treatment of cancer.However,pharmacological and clinical researches indicated that SQ exerts potential therapeutic effect against myocardial ischemia injury.In order to learn more about the specific protective effects and mechanisms of SQ,in this paper,we firstly investigated the pharmacological effects of SQ in vitro and in vivo respectively.Based on the idea of network pharmacology,we constructed Ischemic Heart Disease(IHD)network to examine the connection between SQ and the IHD network and provide systematic evaluation for the cardiovascular pharmacological study of SQ,with the exploring of the relevant pharmacological mechanisms.The main contents were shown as follows:(1)The myocardial hypoxia injury model,oxidative injury model and inflammation model in vitro were built to simulate the different aspects of myocardial ischemia/reperfusion injury.These three cell models were applied to preliminarily study the myocardial protective activity of SQ,DS and HQ.The results showed that,SQ can protect cardiomyocytes from ischemia/reperfusion injury,and its myocardial protection is related to the anti-hypoxia,antioxidant,anti-inflammation activities.DS and HQ presented different effects in these three aspects.(2)The protective activity of SQ against myocardial ischemia/reperfusion injury was validated in vivo.The IHD network was constructed and analyzed with the application of transcriptomics and network pharmacology to explore the mechanism of SQ against IHD.The results presented that SQ achieves the therapeutic effect on IHD mainly through the regulation of following pathways:myocardial energy metabolism,cell adhesion,inflammation,apoptosis,ventricular remodeling,etc.Furthermore,the western blotting analysis validated the regulation on PPAR signaling pathway and apoptosis pathway of SQ,which is consistent with the predicted results.This research provided experimental basis for further comprehending the mechanism of SQ on IHD therapy and expanding its clinical indications.