Effects Of Ginsenoside Rg1 On Autophagy And The Expression Of Beclin-1, LC3 And MTOR In Focal Cerebral Ischemia Reperfusion

ObjectiveTo investigate the effect of ginsenoside Rg1 on mammalian rapamycin(m TOR),microtubule-associated protein 1 light chain 3(LC3),bcl-2 homeodomain protein(Beclin-1)in the parietal lobe of each group was regulated,and then the regulation of ginsenoside Rg1 on autophagy injury after focal cerebral ischemia-reperfusion was also investigated.MethodsThe healthy male rats were randomly divided into four groups: Sham group,model group(IR group),ginsenoside Rg1 group(Rg1 group)and ginsenoside Rg1 plus autophagy inhibitor 3-Methyladenine(3-MA)administration group(RG1+ 3-MA group).The focal cerebral ischemia / reperfusion model in rats was made by reperfusion of middle cerebral artery embolization(MCAO)for 2 hours and reperfusion for 24 hours.Neurological deficit score,brain water content and TTC staining were used to identify whether the model was successful.Transmission electron microscopy was used to observe the autophagy in the rat brain neurons in each group.The expression of m TOR,LC3 and Beclin-1 in parietal lobe of rats were qualitatively and quantitatively detected by immunofluorescence and Western blot.ResultsNeurological deficit scores showed: sham group showed no symptoms of neurological deficit;other groups showed obvious symptoms of neurological deficit.Brain water content measurement results showed: Compared with the sham group,the other groups of brain tissue water content increased significantly.TTC staining results show: sham group without infarction,the other groups visible pale pale infarction.Compared with Rg1 group,IR group and RG1 + 3-MA group obviously pale infarct size increased.Transmission electron microscopy showed no morphological changes of injured neurons in sham group;obvious neuronal autophagy was observed in other groups.Immunofluorescence and Western blotting showed that the IR group and RG1+3-MA group no significant difference.The expression of Beclin-1,LC3 and the expression of Beclin-1,LC3 in sham group were lower than those in sham group,and the expression of Beclin-1,LC3 was the highest and the expression of m TOR was lowest in Rg1 group.ConclusionsGinsenoside Rg1 may upregulate the expression of Beclin-1,LC3 and m TOR in the parietal lobe of the brain,leading to an upregulation of autophagy in brain cells,and thus protect neurons from focal cerebral ischemia-reperfusion in rats.