| ObjectiveWe sought to investigate the protective effects of HO-1C?23(HO-1 fragments lacking 23 amino acids from its C-terminus)on blood-spinal cord barrier(BSCB)after spinal cord injury in rats.MethodsAn adenoviral-HO-1C?23(Ad-GFP-HO-1C?23)was Intrathecal injected at T10 section 7 days before spinal cord injury[1].BBB(Basso Beattie Bresnahan)score[2]Used to evaluate kinematics functional recovery until 28 st days after spinal cord injury;Detection of HO-1 Antibody in slice by immunofluorescence which verified the expression of Ad-GFP-HO-1C?23 in vivo.Evans blue dye,nuclear and cytoplasm extraction and western blot were performed additionally.ResultsCompared with other experimental groups,the BBB motor function scores in nuclear HO-1 group was significantly higher than that in other groups(P <0.05).The nuclear HO-1 group significantly reduced the leakage of the blood-spinal cord barrier in the rat model of spinal cord injury;the expression of Claudin-5 and Occludin was significantly increased in the nuclear HO-1 group.ConclusionAd-GFP-HO-1C?23 group have a better recovery of kinematics function after SCI(spinal cord injury).what ?s more,Ad-GFP-HO-1C?23 enhance the integrity of BSCB(Blood spinal cord barrier)after SCI by unregulated TJ(tight junction)protein. |
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