The Pilot Study Of Correlation Between Different TCM Syndrome Types Of Moderate-to-severe Acne And The Gut Microbiome

Objective To explore the difference in gut microbiome between patients with moderate-to-severe acne(?°-?°)and normal people,and the difference in gut microbiome between acne patients in different TCM syndrome types and normal people.Object In the Department of Dermatology of Wuhan No.1 Hospital from October2017 to February 2018,14 cases of moderate-to-severe acne were13-44 years old,and 14 cases were normal without acne.Methods1.a severe acne questionnaire was designed for the patients' age,sex,occupation,height,weight,BMI,past history,family history,eating habits,location,skin lesion morphology and distribution,clinical symptoms,TCM syndrome type and tongue coating and pulse images,etc.2.the aseptic stool specimens of patients with acne and normal people were collected for about 1g,and DNA in the feces was extracted.The quantitative and quantitative analysis of intestinal microflora was carried out by high throughput sequencing of 16 S r DNA.3.nonparametric_t_test statistical analysis was used to compare the composition,species and quantity of intestinal flora in acne patients and normal subjects.Results1.There was no statistical difference in the sex and age between the patients with acne and the normal control group.The BMI index of the non wet and hot acne patients was higher than the damp heat and the control group,the former was BMI>24,and the latter two were BMI<24.2.A total of 11 phylum,21 classes,28 orders,54 families and 98 genera were detected in 28 specimens.3.No matter in the control group or the acne group,the main dominant flora of the intestinal flora is the bacteriobacteria and the thick wall bacteria,including its subordinate level,the secondary dominant bacteria group mainly with the deformable bacteria gate and its subordinate grade,while the interdependent bacteria gate and the TM7 bacteria gate have not been detected.4.There was no significant difference in the type and quantity of acne group and control group at the level of phylum,class and order.5.At the family level,the Desulfovibrionaceae family(Proteobacteria),Odoribacteraceae(Bacteroides)and Streptococcaceae(Firmicutes)were increased in the acne group.6.At the genus level,Bilophila(proteobacteria),Odoribacter(Bacteroidetes),Streptococcu(Firmicutes),Phascolarctobacterium(Firmicutes),and Clostridium(Firmicutes)were increased in the acne group.7.The acne group was divided into the phlegm damp group and the damp heat group and compared with the control group.Compared with the control group,the abundance of Enterobacteriaceae(proteobacteria) in the control group was higher than that in the phlegm dampness group.The abundance of the alkaloid family and Sutterella bacteria in the phlegm group was higher than that in the control group;the phlegm wet group did not detect the genus Prevotella.8.At the species level,the abundance of melaninogenica in the damp heat group was high,and the abundance in the control group was low,which was 0 in the phlegm dampness group.conclusion1.The intestinal flora of the human body is mainly composed of the bacteriobacteria,the thick wall and the deformable bacteria.It does not contain the interdependent bacteria gate and the TM7 bacteria gate,which has nothing to do with the acne.2.The abundance of Enterobacteriaceae in acne patients is low.Enterobacteriaceae belongs to proteobacteria.It can synthesize essential organic acids and some neutral solvents.3.In the acne classification,the non humid heat deposits(phlegm wet blood coagulation and non adjustment)are more associated with obesity,and the abundance of the genus pervo is significantly lower in the body,and the genus perivium is under the pseudo bacilli gate,and the bacilli have been proved to be decreased in the obese patients.4,obesity can affect the intestinal flora.