| ObjectiveWith the continuous development of cell biology and tissue engineering,open up new areas for the treatment of bone defects.That the placement of membrane defects in the bone as a mechanical barrier,the surrounding connective tissue can be blocked at the defect,resulting in a special regeneration space,the preponderance of bone-forming cells to produce new bone to achieve the purpose of promoting bone healing,inducing membrane technology is also Based on this principle.Select the ideal combination of membrane and subretinal materials to repair bone defects is the key.This project intends to build biologically active tissue similar to human periosteum periosteum,bone marrow mesenchymal stem cells(BMSCs)and healthy full-term pregnant women amniotic acellular amniotic membrane(HAAM)composite made in vitro observation of tissue engineering periosteum Biological activity,and testing their compatibility,in order to lay the foundation for the repair of large bone defects in vivo.Due to the large segment of the repair of bone loss is particularly difficult[1],animal experiments in tissue engineering periosteal technology is expected to become a good recipe for clinical treatment of bone defects.The construction of tissue engineering periosteum,the related research for the development of bone tissue engineering and treatment provides a new opportunity,has gradually become a hot spot for the manufacture of bony periosteum defect site provides the possibility.MethodIn Vitro Experimental Study of Human Acellular Amnion and Rabbit Bone Marrow Mesenchymal Stem Cells in Constructing Tissue Engineered Periosteum.The structure of human acellular amniotic membrane was observed by HE staining and scanning electron microscopy(SEM).MTT showed the growth of bone marrow mesenchymal stem cells on human acellular amniotic membrane.Calcium nodule staining(Alizarin red staining,Von kossa staining),ALP(alkaline phosphatase)test showed that composite tissue engineering periosteum can produce osteoblasts.In vivo study of tissue engineering periosteum derived from human amniotic membrane and rabbit bone marrow mesenchymal stem cells.By inserting the tissue-engineered periosteum constructed in vitro into the large bone defect of rabbits,the growth of bone in the bone defect was examined by imaging.To avoid the large bone defect non-healing and long treatment cycle characteristics to overcome the shortcomings,the treatment evaluation more comprehensive.ResultIn Vitro Experimental Study of Human Acellular Amnion and Rabbit Bone Marrow Mesenchymal Stem Cells in Constructing Tissue Engineered Periosteum.1 by physical and chemical methods such as human acellular amniotic membrane,the surface without cell residue,without destroying the structure and composition of fibrous tissue was reticular staggered distribution of three-dimensional space structure.Through the detection of bone marrow mesenchymal stem cells in human acellular amniotic membrane growth curve,indicating that the experimental group of proliferating cells more than the control group.Rabbit bone marrow-derived mesenchymal stem cells(BMSCs)induce osteoblasts after osteogenic induction.Alkaline phosphatase(ALP)and calcitonin nodules are the markers of osteoblasts.ALP and calcitonin can be detected Festival content.In vivo study of tissue engineering periosteum derived from human amniotic membrane and rabbit bone marrow mesenchymal stem cells.Statistical analysis showed that the bone defect recovery in rabbits after periosteal implantation was better than that in rabbits without periosteum.After imaging examination,rabbits’bony defects implanted with tissue engineering periosteum healed.ConclusionIn this study,based on the treatment of large segmental defects in rabbits,we established a rabbit model of bone defects and evaluated the biological characteristics of tissue-engineered periosteum in vitro.The treatment of large segmental defects in rabbits was completed by imaging evaluation.Compared with the traditional methods,the system has more advantages in treatment methods,the evaluation index is rich and comprehensive to make up for the deficiencies,which is beneficial to improve the accuracy and repeatability of the therapeutic evaluation results.It is expected to provide a new type of clinical treatment for patients with large bone defects The treatment. |
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