LncRNA LOC283194 Regulates Cell Cycle And Apoptosis In Prostate Cancer Cell Lines And Predicts Poor Prognosis In PCa Patients

Objective: To investigate the effect of the prostate cancer-up-regulated lnc RNA LOC283194 on the cell function of prostate cancer(PCa)cell lines,and to analyze the clinical significance of measurement of LOC283194 content in cases of human PCa.Materials and methods: Quantitative real-time polymerase chain reaction(q RT-PCR)was used to demonstrate the expression of LOC283194 in 64 pairs of PCa tumor and adjacent non-tumor tissues,and in PC3,DU-145 and WPMY-1 cell lines.Cell cycle and apoptosis were assessed using flow cytometry in PC3 and DU-145 cell lines following suppression of LOC283194 by si RNA.The influence of LOC283194 on the viability and proliferation these cell lines were evaluated with Cell Counting Kit-8(CCK-8)assay and cell cloning experiments.The correlation of LOC283194 expression with clinic-pathologic characteristics and prognosis of PCa patients was assessed by Log-Rank test and Cox proportional hazards model.Results: The expression of LOC283194 was significantly higher in human PCa tissues compared with that in adjacent non-tumor tissues,as well as in PCa cell lines relative to a normal prostate cell line.We further demonstrated that LOC283194 increased the expression of cell cycle regulatory protein Cyclin D1,induced cell cycle arrest in S phase and reduced cell cycle arrest in G0/G1 phase.In addition,our study showed that knockdown of LOC283194 induced apoptosis of PCa cell lines.Besides,the high expression level of LOC283194 was associated with advanced features and poor prognosis of PCa.The multivariate analysis confirmed LOC283194 expression was an independent prognostic factor for PCa.Conclusion: LOC283194 induced S phase PCa cells and inhibit apoptosis of PCa cells,and it may represent a prognostic biomarker in PCa diagnosis and treatment.