In Vitro Antibacterial Effect Of The Waixizhiyang Prescription Of Different Concentrations On The Staphylococcus Aureus

Objective: In vitro antibacterial effect of the WaiXiZhiYang prescription of different concentrations on the staphylococcus aureus.To provide reference for clinical sitting bath concentration.Methods: In accordance with the relevant standards of liquid dilution of staphylococcus aureus in vitro bacteriostasis experiment,wash outside the anus bowel division itching party 1g/ml,more times than diluted into 1/2,1/4 1/8,1/16,1/32 1/64,1/128,1/256 as much as nine dilution degrees,join adjust good bacteria liquid.After 12 h at37?cultivate macroscopic observation results.The bacterial growth was observed after 12 h was cultured with 100 ul bacteria of 3 concentrations of bacterial growth.The minimum drug concentration that can inhibit bacterial growth is the MIC value of the drug.Results: After 12 h after 37 ? training,solution concentration gradually reduce,bacteriostatic effect,therefore,visible to the naked eye glass tube bacteria liquid gradually feculence,choose the naked eye to see the growth of the sterile tube,pipe and tube 5,4,3and 10 tube as controlled on line training at the same time,to observe the antibacterial.12 h line 37 ? after training,the third and fourth tube,solution concentration,respectively is1/4and 1/8 at this time no bacterial growth in a petri dish,drugs has reached the bacteriostasis,therefore,to determine 1/8 to inhibit the growth of staphylococcus aureus minimum drug concentration,namely the drug MIC value is 1/8.Conclusion: In vitro antibacterial experiments of staphylococcus aureus were studied by using different concentrations.prove that wash outside itching has retarding effect to staphylococcus aureus,and patients to take a bath when the drug concentration of1/8(125mg/ml)can have good antibacterial effect,when less than1/8(125mg/ml)concentration may bacteriostasis abate or invalid.sitz bath concentration provides theoretical basis for traditional Chinese medicine.