Studies On Diterpenoid Alkaloids From Aconitum Sinchiangense.W.T.Wang

Objective:Investigation to the diterpeniod alkaloids from Aconitum sinchiangense W.T.Wang.Including the saparation and structural identification.Methods:1)Extract with chloroform-methanol 4:1 under cold extraction method and futher extract by ethyl acetate after evaporation.2)Saparate and purify compounds by using the silica gel column chromatography,preparative Sephadex LH-20 column,preparative TLC,preparative-HPLC chromatography.3)The chemical structures were identified by ESI-MS and 1D,2D NMR which includes the ¹H-NMR、¹³C-NMR、HMBC、HSQC、¹H-¹H COSY,NOESY,DEPT,and MS datas.Results:Nine diterpenoid alkaloids were isolated from Aconitum sinchiangense W.T.Wang by using a variety of modern chemical separation and analysis methods,including a new C₂₀-diterpenoid alkaloid:sinchianine（1）,together with three known C₂₀-diterpenoid alkaloids:12-acetyl-12-epi-napelline（2）,12-epi-napelline（3）,songorine（4）,and five known C₁₉-diterpenoid alkaloids,neoline（5）,talatisamine（6）,14-O-acetylsenbusine A（7）,benzoylaconine（8）and aconitine（9）.Conclusion:All compounds were isolated from this plant for the first time.The new compound 1 is a rare denudatine type C₂₀-diterpenoid alkaloid.This survey enriched the chemical constituents of Aconitum sinchiangense W.T.Wang.And it provided an important basis for the further activity screening research and the deep development and utilization of this characteristic plant resource in Xinjiang.