Effects Of Simvastatin Sustained-Release Microspheres On Bone Formation Of Bone Tissue Engineering In Vitro

BackgroundOral implant repair is a method mainly used to treat dentition defects and edentulous jaw,and is currently the choice of more and more patients in clinical practice.Oral implant repair has certain requirements for the jaw bone mass of the patient.However,the patient often has insufficient bone mass of the jaws.It is necessary to increase the bone mass through surgery to create conditions for implant repair.Therefore,choosing the source of bone replacement material with a short time to increase bone mass and being safe and reliable has become a research hotspot to solve this problem.Mundy et al.found that simvastatin has such bone-enhancing efficacy.PurposeBased on the research results of Porous Hydroxyapatite(HA)ceramic scaffolds with independent intellectual property rights in the previous period,this project continues to explore the ossification mechanism of porous HA ceramic scaffolds.And comparison of bone formation performance of bone tissue engineering constructed with the above three different conditions.Methods:1.Nanoscale liposomes containing simvastatin were prepared by film dispersion method,and the particle size and polydispersity index were measured by a Malvern particle size analyzer.2.The result of a slow release experiment performed under a dialysis apparatus was measured with a UV2100 UV-Vis spectrophotometer.The nanoliposome and simvastatin pure drug solutions were tested for release at 1d,2d,3d,5d,7d,and 14 d,and the release curves were visualized to reveal the release of the SIM release microspheres at these time points.3.The transcriptome gene levels of human placenta-derived mesenchymal stem cells(PMSCs)and novel porous hydroxyapatite(HA)ceramic scaffolds were tested in tissue engineering cultures constructed under three different conditions for 7 days,14 days,and 21 days.The results of the gene level test were then reverse-validated using western blot and fluorescence quantitative PCR.Results:1.The average diameter of the SIM nano-liposomes is less than 100 nm,and the polydispersity coefficient PDI value is 0.131,indicating that the SIM nano-liposomes are small in diameter and uniform in size.2.Sustained release experiments demonstrated that the SIM nano-liposomes can sustain sustained release of the drug for at least 14 days while the SIM-only solution was almost completely released in just 3 days.3.The 3.3?mol/L SIM selected in this experiment in a total of 9 gene expression results for 3 genes of the 3 experimental groups,there were 5 in the highest expression levels of the 3 genes of BMP-2,BMP-4,and VEGF is blank group,while only two were in the experimental group and the experimental control group.4.In the process of osteogenic differentiation in three groups,VEGF showed higher expression later than BMP-2 and BMP-4.