| Objective: To investigate the effect of AZ31 magnesium alloy scaffolds on osteoblastic cell function.Meanwhile,the calcium phosphate coating was prepared by chemical deposition method on AZ31 magnesium alloy scaffolds and its influence on the corrosion resistance and biocompatibility of AZ31 magnesium alloy scaffolds were studied.Methods:(1)Influence of magnesium alloy scaffolds on the adhesion,proliferation and mineralization abilities of osteoblasts: The associated effects of the magnesium alloy scaffolds on osteoblastic cell function,involving cell adhesion,cell proliferation,and mineralization,were investigated using calcein staining,MTT assay and ambramycin staining respectively.Pure magnesium scaffolds were used as control group.(2)Influence of calcium phosphate coating(Ca-P coating)on the corrosion resistance and biocompatibility of magnesium alloy scaffolds: The morphology and composition of the Ca–P coating were characterized by scanning electron microscopy and energy dispersive spectroscopy.The corrosion behavior of the Ca-P coating was evaluated by a static immersion test and the effects of the Ca–P coating on biocompatibility were also investigated by in vitro cell experiments.Results:(1)Influence of magnesium alloy scaffolds on the adhesion,proliferation and mineralization abilities of osteoblasts: The number of cells adhered to the surface of the magnesium alloy scaffolds were significantly more than that to the surface of the pure magnesium scaffolds at both 12 and 24 h(P < 0.05);the results of proliferation assays showed that noticeably higher absorbance was observed on the magnesium alloy scaffolds than on the pure magnesium scaffolds after 3 and 5 days culture(P < 0.05);the number and area of mineralized nodules formed on the surface of the magnesium alloy scaffolds was greater than that on the surface of the pure magnesium scaffolds after 21-day culture(P < 0.05).(2)Influence of calcium phosphate coating(Ca-P coating)on the corrosion resistance and biocompatibility of magnesium alloy scaffolds: The Ca–P coating reduced the corrosion rates of the AZ31 magnesium alloy scaffolds.Cell experiments showed significantly good adherence and high proliferation on the Ca–P-coated AZ31 magnesium alloy scaffolds than those on the uncoated AZ31 magnesium alloy scaffolds(P < 0.05).The blood cell aggregation tests showed that the Ca–P-coated AZ31 magnesium alloy scaffolds had decreased the blood cell aggregation compared to the uncoated AZ31 magnesium alloy scaffolds.Conclusions:(1)The magnesium alloy scaffold with an excellent bioactivity can promote the adhesion,proliferation and mineralization abilities of osteoblasts.(2)The Ca–P coating not only slowed down the corrosion rate of the AZ31 magnesium alloy scaffolds,but also improved its biocompatibility. |
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