The Effect Of TLR4?MyD88?iNOS On The Occurrence And Metastases In Prostate Cancer

Objective:Prostate cancer is one of the most common tumors in the male urogenital tumors.The pathogenesis is still uncertain.It's symptom is not obvious and particular in the early stage.The curative effect of the Prostate cancer is unsatisfactory because it was been discovered in the last stage mostly.TLR4,MyD88 is an important pathways in inflammation,TLR4 activate the NF-?B pathways by the TLR4/MyD88 pathways or another no MyD88 pathways.The iNOS,TNF-a,IL-1 and other cytokines can be secreted by NF-?B pathways to regulation of the inflammation response and the cell cycle.Nowadays,the relationship between inflammation and tumor has attracted more and more attention,and the relationship between TLR4,MyD88,iNOS and various tumors has also been extensively studied.A lot of studies shown that the TLR4,MyD88,iNOS pathways are closed to the development of the tumor,such as the Breast cancer,lymphoma.It plays a significant role in the regulation of the tumor cell cycle.It is always uncertain that the relation between the TLR4,MyD88,iNOS and the prostate cancer nowadays.In our study,we detected the expression of the TLR4,MyD88,iNOS and to explore its effect on the occurrences and metastases of the prostate cancer.Methods1 TLR4 mRNA expression were determined by RNAscope ISH in prostate cancer tissues from 40 patients.The 20 benign prostatic hyperplasia tissues and 28 prostatitis tissues as control.Expression of TLR4 mRNA was correlated with clinicopathogic parameters of the prostate cancer.2 TLR4,MyD88,iNOS expression were determined by immunohistochemistry in prostate cancer tissues from 40 patients.The 20 benign prostatic hyperplasia tissues and 28 prostatitis tissues as control.Expression of these proteins was correlated with clinicopathogic parameters of the prostate cancer.Result1 TLR4 mRNA expression in prostate cancer were higher than that in benign prostatic hyperplasia(p<0.05),TLR4 mRNA expression in prostatitis were higher than that in benign prostatic hyperplasia(p<0.05),TLR4 mRNA expression in prostate cancer,prostatitis were similarly(p>0.05),and the expression increased in the metastatic prostate cancer compared to non-metastatic prostate cancer(p<0.05).The TLR4 mRNA expression levels increased in high Gleason score prostate cancer(p<0.05),but had no connection with the age and serum PSA levels of patients(p>0.05).2 TLR4 protein expression in prostate cancer were higher than that in benign prostatic hyperplasia(p<0.05),TLR4 protein expression in prostatitis were higher than that in benign prostatic hyperplasia(p<0.05),TLR4 protein expression in prostate cancer,prostatitis were similarly(p>0.05),and the expression increased in the metastatic prostate cancer compared to non-metastatic prostate cancer(p<0.05).The TLR4 expression levels increased in high Gleason score prostate cancer(p<0.05),but had no connection with the age and serum PSA levels of patients(p>0.05).3 MyD88 protein expression in prostate cancer,benign prostatic hyperplasia and prostatitis were similarly(p>0.05),and the expression had no connection with the stage of the cancer(p>0.05).The age and serum PSA levels of patients had nothing to do with the MyD88 expression in prostate cancer(p>0.05).4 iNOS protein expression in prostate cancer were higher than that in benign prostatic hyperplasia and prostatitis(p<0.05),iNOS protein expression in benign prostatic hyperplasia and prostatitis were similarly(p>0.05),and the expression decreased in the metastatic prostate cancer compared to non-metastatic prostate cancer(p<0.05).The iNOS expression levels increased in high Gleason score prostate cancer(p<0.05),but hsd no connection with the age and serum PSA levels of patients(p>0.05).5 TLR4 mRNA expression in prostate cancer were closed to TLR4 protein expression in prostate cancer(p<0.05).TLR4 protein expression in prostate cancer were closed to iNOS protein expression in prostate cancer(p<0.05).TLR4 protein expression in prostate cancer had nothing to do with MyD88 protein expression in prostate cancer(p>0.05).iNOS protein expression in prostate cancer had nothing to do with MyD88 protein expression in prostate cancer(p>0.05).Conclusion1 The expression of TLR4 and iNOS in prostate cancer affects the occurrence of prostate cancer.2 The expression of TLR4 and iNOS in prostate cancer affects the metastasis of prostate cancer.3 Expression of TLR4 mRNA is related to the expression of TLR4 protein in prostate cancer.Expression of TLR4 protein is related to the expression of iNOS protein in prostate cancer.