The Development Of Mulit-Functional And Controllable Nano-Probe And Its Application In Tumor Cell Targeting Recognition And Photothermal Therapy

Cancer is well recognized as a major threat to human health all around the world.Studies on this disease have been focused on the pathogenesis of cancer development and the counter measures,development of techniques for analyzing tumor cells and tumor markers,and investigation of novel,highly effective and highly specific anticancer drugs.In this study,we synthesized two novel photothermal nanomaterials and tested their performance in photothermal therapy of tumor cells.1.In this work,we synthesized the T Type DNA-Pt@Au nanoring probe and used it for fluorescence imaging and photothermal therapy of CCRF-CEM tumor cells.The new probe integrated a dual-DNA aptamer design,which improved specificity of the probe.Activation of the DNA adaptor relies on the photothermal effect of the Pt@Au nanoring,so that the probe works only after irradiation with near-infrared laser,which improves operability of the probe.We tested the performance of the new probe using CCRF-CEM as the target cells and MCF-7 cells as control,and the result suggested good operability and satisfactory photothermic therapeutic effect.2.In addition,we also developed a CuS@Au system for controllable and targeted delivery of drugs,which was tested for photothermal therapy of U87-MG tumor cells.This CuS@Au system uses a hollow and pored gold nanocage as carrier,which itself showed photothermal effect.The nanocage was loaded with CuS photothermal quantum dots or CdTe fluorescence quantum dots,encapsulated with DNA,and its surface was decorated with ?v?3 integrin antibody that granted the probe specificity for U87-MG tumor cells.Test results suggested high specificity of the probe for the targeted tumor.By adjusting the parameters of near-infrared laser,the probe effectively released the CuS photothermal quantum dots or CdTe fluorescence quantum dots in the tumor cells due to photothermal effect upon irradiation.The system showed good specificity for U87-MG tumors and exerted good photothermal therapeutic effect.