| Background: Allergic purpura is also called Henoch Schonlein purpura,HSP.It is more common in children caused by a variety of reasons for the non thrombocytopenic hemorrhagic disease,and a kind of allergic disease of microvascular.The disease often occurs in school-age children,boys slightly more than girls,more often in the autumn and winter,is usually self limiting disease,the prognosis is almost satisfying,but about 15% cause the kidney damage causing severe effects on the quality of life of children,and 8% of which can progress to kidney failure.According to the different target organ damage HSP can be divided into five types: 1,simple skin purpura,2,joint type allergic purpura,3,abdominal type allergic purpura,4,renal allergic purpura,5 mixed type allergic purpura(two or more kinds of types exist at the same time).At present,the etiology of HSP in children at home and abroad is not clear in general,and is related to a variety of bacteria,viral infections,drugs,special food and so on.Humoral immunity is the main cause of the disease,among which Ig A mediated humoral immunity is primary,and the related cytokines,T lymphocyte dysfunction and inflammatory mediators are also involved in the process of disease.Many scholars believe that the infection of many pathogenic microorganisms plays an important role in the pathogenesis of HSP in children,and the infection of children is significantly higher than that of adults.Related bacteriology study found that Streptococcus infection,Helicobacter pylori(HP)is closely related to the incidence of HSP in children.It has been reported that skin purpura,proteinuria and decreased serum complement levels may occur after streptococcal infection.The pathological diagnosis of renal biopsy suggests that it is purpura nephritis.Some domestic scholars study shows that the incidence of HP infection in children with allergic purpura is closely related with the incidence of abdominal typeallergic purpura;recent studies show that HPV-B19 infection and the incidence of anaphylactoid purpura has certain correlation,which may be related to the pathogen of children allergic purpura.Other mycoplasma pneumonia,Coxsackie virus,EB virus,hepatitis B vaccine,hepatitis A vaccination,hepatitis A vaccine,hepatitis B vaccine,rubella vaccine,measles rubella combined anti allergic purpura in children were reported to cause virus vaccine,but the specific mechanism fails to clearly articulate.This study detect TORCH in patients with different types of children allergic purpura T(Toxoplasma gondii)R(Rubella virus)of Toxoplasma gondii,rubella virus,cytomegalovirus(C Cytomegalo Virus),H(Herpes.Virus)of herpes simplex virus type Ⅰ/Ⅱ,O(Others)and other viral infections,and compared with normal control group,in order to investigate the relationship between TORCH infection and Henoch Schonlein purpura in children,so as to provide a theoretical basis for the prevention and treatment of HSP.Objectives: This study detected TORCH in patients with different types of children allergic purpura T(Toxoplasma gondii)R(Rubella virus)of Toxoplasma gondii,rubella virus,cytomegalovirus(C Cytomegalo Virus),H(Herpes simplex virus)of herpes simplex virus type Ⅰ/Ⅱ,O(Others)and other viral infections,and compared with normal control group,the incidence of association in order to investigate the TORCH infection and HSP in children,so as to provide theoretical basis for prevention and treatment of HSP.Methods:1 Research objects: From January 1,2016 to December 31 2016 in our hospital outpatient and inpatient diagnosed 277 cases of children with Henoch Schonlein purpura.According to different clinical types are divided into simple type A(82 cases of skin purpura),type B(joint type skin purpura with joint symptoms)36 cases,type C(abdominal skin purpura with gastrointestinal symptoms)72 cases,type D(kidney positive urine protein,occult blood more than 3)45 cases,type E(mixed more than 2 kinds of symptoms)42 cases.Examined by TORCH T(Toxoplasma gondii)R(Rubella virus)of Toxoplasma gondii,rubella virus,C(Cytomegalovirus),H(Herpessimplex virus)of herpes simplex virus type Ⅰ/Ⅱ,O(Others)and other viral infections,and compared with normal control group,in order to investigate the pathogenesis of TORCH associated with a sense of children with allergic purpura,so as to provide theoretical basis for prevention and treatment.2 Torch: At the same time,TORCH antibody Ig G and Ig M were detected by ELISA method.All the subjects used the same reagent from the same origin,and the specific operation procedures were strictly carried out according to the instructions.According to TORCH-Ig M,TORCH-Ig G ELISA kit instructions to determine the effectiveness of the results.Judge the result of the specimen:Positive samples: the OD values and the critical ratio of serum OD value is greater than or equal to 1.1.Negative: OD value and serum specimens of critical od ratio is less than or equal to 0.9.Suspicious: OD value and serum specimens in the critical OD value is between 0.9 and 1.1.3 Statistical analysis:All the statistical data are calculated by SPSS20.0software package.The count data is expressed in percentage and constituent ratio,and the measurement data is expressed by mean standard deviation.The difference of antibody resistance rate between Henoch Schonlein purpura group and control group was compared by chi square test of completely randomized and designed in four compartment table data.Results: Influence of various viral infections in TORCH examination on the classification of children with Henoch Schonlein purpura.Among the 82 patients with type A simplex(only skin purpura),4 cases were positive for HSV-I-Ig M,the positive rate was 4.88%,and 19 cases were positive for HSV-I-Ig G,the positive rate was 23.17%.5 cases were positive for HSV-II-Ig M,the positive rate was 6.10%.9 cases were positive for HSV-II-Ig G,the positive rate was 10.98%.6 cases were positive for RV-Ig M,the positive rate was 7.32%,3 cases were positive for RV-Ig G positive,the positive rate was 3.66%.5 cases were TOX-Ig M positive,the positive rate ofwas 6.10%,12 cases were positive for TOX-Ig G,the positive rate was14.63%,4 cases were CMV-Ig M positive,the positive rate was 4.87%,7 cases were positive for CMV-Ig G,the positive rate was 8.53%,type B(joint type skin purpura with joint symptoms)were found in 36 cases,3 cases were HSV-I-Ig M positive,the positive rate was 8.33%,6 cases were HSV-I-Ig G positive,the positive rate was 16.67%.3 cases were positive for HSV-II-Ig M,the positive rate was 8.33%.5 cases were positive for HSV-II-Ig G,the positive rate was 13.89%.2 cases were positive for RV-Ig M,the positive rate was 5.56%,3 cases were positive for RV-Ig G,the positive rate was 8.33%.3cases were positive for TOX-Ig M,the positive rate was 8.33%,4 cases were Ig G positive,the positive rate was 11.11%,4 cases were positive for CMV-Ig M,the positive rate was 11.11%,7 cases were positive for CMV-Ig G,the positive rate was 19.44%,type C(abdominal type purpura with gastrointestinal symptoms)was found in 72 cases,7 cases were positive for HSV-I-Ig M,the positive rate 9.72%,12 cases were positive for HSV-I-Ig G,the positive rate was 16.67%.4 cases were positive for HSV-II-Ig M,the positive rate was 5.56%.7 cases were positive for HSV-II-Ig G,the positive rate was 9.72%.3 cases were positive for RV-Ig M,the positive rate was 4.17%,3 cases were positive for RV-Ig G,the positive rate was 4.17%.3 cases were positive for TOX-Ig M,the positive rate was 4.17%,5 cases were positive for TOX-Ig G,the positive rate was 6.94%,7 cases were positive for CMV-Ig M,the positive rate was 9.72%,15 cases were positive for CMV-Ig G,the positive rate was 20.83%,type D kidney(positive urine protein,occult blood more than 3)was found in 45 cases,3 cases were positive for HSV-I-Ig M,the positive rate was 6.67%,7 cases were positive for HSV-I-Ig G,the positive rate was 15.56%.4 cases were positive for HSV-II-Ig M,the positive rate was8.89%.6 cases were positive for HSV-II-Ig G,the positive rate was 13.33%.3cases were positive for RV-Ig M,the positive rate was 6.67%,5 cases were positive for RV-Ig G,the positive rate was 11.11%.5 cases were positive for TOX-Ig M,the positive rate was 11.11%,6 cases were positive for Ig G,the positive rate was 13.33%,4 cases were positive for CMV-Ig M,the positiverate was 8.89%,9 cases were positive for CMV-Ig G,the positive rate was20%,type E mixed(more than 2 kinds of symptoms exist)were found in 42 cases,3 cases were positive for HSV-I-Ig M,the positive rate 7.14%,8 cases were positive for HSV-I-Ig G,the positive rate was 19.05%.4 cases were positive for HSV-II-Ig M,the positive rate was 9.52%.6 cases were positive for HSV-II-Ig G,the positive rate was 14.29%.2 cases were positive for RV-Ig M,the positive rate was 4.76%,3 cases were positive for RV-Ig G,the positive rate was 7.14%.3 cases were positive for TOX-Ig M,the positive rate was 7.14%,4 cases were positive for TOX-Ig G,the positive rate was 9.52%,6 cases were positive for CMV-Ig M,the positive rate was 14.29%,8 cases were positive for CMV-Ig G,the positive rate was 19.05%.Conclusion:1 The positive rates of HSV-I-Ig G and CMV-Ig G in children with Henoch Schonlein purpura were significantly higher,suggesting that HSV and CMV may be related to the pathogenesis of Henoch Schonlein purpura.2 In children with Henoch Schonlein purpura,Ig G is higher than the positive rate of Ig M,suggesting that the above types of virus long-term infection and allergic purpura correlation is stronger than the recent infection.3 There were no significant differences in HSV-II,RV and TOX related antibodies among children with Henoch Schonlein purpura,indicating that there was no correlation between the above type of viral infection and Henoch Schonlein purpura in children.4 In children with various types of HSP,HSV-I virus infection is associated with an increased incidence of HSP,and CMV virus infection is associated with an increased incidence of type BCDE HSP. |
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