Study On The Preparation And Properties Of Chitosan-Nano Silver-Rubber Latex Composite Antibacterial Material

With the development of modern medical technology,medical intervention catheters have been widely used.However the obvious problem of catheter is that the catheter can easily lead to infection in clinic,which means more pain and more burdens.Therefore,Synthesizing secure and efficient antibacterial polymer materials was one of the most active domains in biomedicine macromolecule research.In this article,silver nanoparticles were prepared through Ultraviolet?UV?Irradiation.Antibacterial latex was prepared used this silver nanoparticles as antibacterial agent by solution blending method and self-assembly method.This work has laid the foundation for preparing antibacterial medical catheter.Low molecular weight chitosan modified silver nanoparticles?LMCS-AgNPs?were prepared through Ultraviolet?UV?Irradiation with low molecular weight chitosan?LMCS?prepared by H₂O₂ oxidative degradation as the reductant and surface modification agent.The antibacterial latex was prepared by solution blending method and self-assembly method.The silver nanoparticles and antibacterial natural latex were characterized using ultraviolet-visible spectroscopy?UV-Vis?,fourier transform infrared?FTIR?,X-ray diffraction?XRD?analysis,laser particle size analyzer,transmission electron microscopy?TEM?and scanning electron microscopy?SEM?.The antibacterial properties of silver nanoparticles were investigated by micro-broth dilution method and inhibition ring test.The antibacterial properties of antibacterial latex were investigated by plate colony-counting methods,fluorochrome stain assay and inhibition ring test.The main results are summarized as follows:?1?LMCS-Ag NPs were spherical or nearly spherical with average diameter of 15-35 nm.LMCS is effective on preventing from aggregation of silver nanoparticles.LMCS-AgNPs shows best antibacterial property at the rate of AgNO₃ and low molecular chitosan is 0.5:9.5.?-galactosidase activity assay and E.coli morphology characterization?SEM?indicated that AgNPs could destroy the integrality of the cytoplasmic membrane,leading to leakage of cytoplasm.?2?Blending with LMCS-AgNPs modified the rubber latex.The LMCS-Ag NPs/NR antibacterial agent was uniformly dispersed in rubber latex and combined tightly with the natural latex.Because of Ag⁺release,the LMCS-Ag NPs/NRclayshadexcellentantibacterialproperties.LMCS-Ag NPs/NR could kill completely the bacteria on the surface of naturel rubber with the LMCS-AgNPs concentration exceeds 500?g/mL.?3?Based on the electrostatic force driven layer-by-layer self-assembly,the LMCS-Ag NPs were further assembled with GO to form antibacterial coating?LMCS-AgNPs/GO?on the surface of naturel rubber.80%H₂SO₄ can impart the naturel rubber increased surface roughness and the improved hydrophilicity by the introduction of polar functions containing oxygen.UV-vis showed that the self-assembly process was regular,and SEM analysis indicated that LMCS-Ag NPs were uniformly embedded into the coating.Plate colony-counting methods and fluorochrome stain assay indicated that the LMCS-Ag NPs/GO antibacterial coating could kill completely the bacteria on the surface of naturel rubber with the the number of self-assembly greater than or equal to 1.Inhibition ring test indicated that the diameter of the inhibition ring exceed antibacterial standard of 7 mm with the the number of self-assembly exceeds 1.