| Objective To explore the role of CaV1.2 protein in sevoflurane anesthesia in rats.Methods Rats were randomly divided into 5 groups(n=8)using a digital table method: control group,sevoflurane 1h group(sevo 1h),sevoflurane 2h group(sevo 2h),sevoflurane 3h group(sevo 3h),sevoflurane 4h(sevo 4h).The control group inhaled oxygen composite air.The rest of the groups were inhaled with 2sevoflurane-induced anesthesia,and then maintained with 1.5 MAC sevoflurane for 1 h,2 h,3 h,and 4 h.The time when the righting reflexes disappeared in each group under sevoflurane anesthesia was recorded.The vital signs of the rats were monitored and recorded at three time points before anesthesia(T1),during anesthesia(T2)and at the end of anesthesia(T3).After the end of anesthesia,0.5ml of blood was collected from the apex of the heart for blood gas analysis,and the brain was decapitated for protein level detection.Agonist group: The dose-effect relationship analysis was performed.FPL64176 was divided into three concentrations of 0.1umol,1umol,and 10 umol through the cerebral ventricle for administration of anesthesia.The time of anesthesia induction(disappearance of righting reflex)was recorded,and routine recording was monitored.After the end of anesthesia,vital signs were removed and the brains were decapitated for Western blot and immunohistochemical analysis.Inhibitor group: randomly divided into 3 groups(n = 8): control(containing DMSO),sevo 4h,isradipine group,lateral ventricle catheter administration,analgesia sedation score.ResultsHE staining showed that about 1.5 MAC sevoflurane had no effect on rat neural cells;there was no difference in the time of righting reflex disappearance in each group;Western blot results showed that compared with control group,sevo 1h,sevo 2h,sevo 3h,sevo The expression of Cav1.2 protein decreased gradually at 4h,and decreased significantly at sevo 3h and sevo 4h(P<0.05),and there was a significant difference between sevo 4h and sevo 3h(P<0.05).Immunohistochemical results showed that : Compared with control,the expression of CaV1.2 positive cells decreased in turn was statistically significant(P<0.05).Excitation unit: Compared with the control group,the time for the disappearance of righting reflex in the FPL64176 group was prolonged(P<0.05).Western blot results showed that compared with the control group,CaV1.2 protein expression in the FPL64176 group rats There was a significant difference(P<0.05).The expression of CaV1.2 protein in sevo 4h +FPL64176 group was significantly higher than that in sevo 4h group(P<0.05).The results of immunohistochemistry showed that the expression of CaV1.2 positive cells in the FPL64176 group was significantly higher than that in the control group(P<0.05).The expression of CaV1.2 cells was higher in the sevo 4h + FPL64176 group than in the sevo 4h group.Statistical significance(P<0.05).Inhibitor group: Western blot results showed that the expression of CaV1.2 protein in the isradipine group was significantly lower than control(P<0.05).The results of immunohistochemistry showed that compared with control,there was a significant difference in CaV1.2-positive cells in the isradipine group(P<0.05).Compared with control,isradipine group had a statistically significant decrease in analgesic sedation score(VOCR and TRP scores)(P<0.05).Conclusions CaV1.2 protein is gradually decreased under sevoflurane anesthesia.The induction time of CaV1.2 is significantly prolonged after Cav1.2 expression is enhanced.CaV1.2 inhibitionhas sedative analgesic effect on rats. |
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