MiR-27a-3p Promotes The Malignant Phenotypes Of Osteosarcoma By Targeting Ten-eleven Translocation 1

Osteosarcoma has become one of the most common primary malignant tumors in the children and adolescents.Although increasing evidence indicates that miRNAs play important roles in the tumorigenesis of osteosarcoma,the expression of miR-27a-3p and its effects in osteosarcoma is still very poor.In the present study,our data showed that the expression of miR-27a-3p in osteosarcoma cell lines was significantly higher than that in hFOB 1.19 cell(p<0.01).In order to explore the mechanism of miR-27a-3p in the tumorigenesis and progression of osteosarcoma,the expression of miR-27a-3p was inhibited by the transfection of miR-27a-3p inhibitor in MG-63 cells.Results showed that the cell proliferation ability decreased significantly(p<0.01),the number of apoptotic cells increased significantly(p<0.01),and the number of cells passing through transwell membrane was significantly reduced in the miR-27a-3p inhibitor transfection group(p<0.01).At the same time,the expression of E-cadherin and ?-catenin was significantly upregulated in the miR-27a-3p inhibitor transfection group(p<0.01),while the expression of vimentin was significantly downregulated in the miR-27a-3p inhibitor transfection group(p<0.01).Our results also showed that the expression of ten-eleven translocation 1(TET1)mRNA in osteosarcoma cell line was significantly downregulated compared with hFOB 1.19 cells(p<0.01).Luciferase reporter system was used for further verify the relationship between miR-27a-3p and its target gene.The results showed that miR-27a-3p could recognize with TET1 3'UTR.The expression of TET1 protein increased significantly in the miR-27a-3p inhibitortransfection group.The results from CCK-8 analysis,FCM assay and transwell invasion analysis showed that the inhibition of mi R-27a-3p could reverse the biological effects induced by inhibiting the expression of TET1.Taken together,miR-27a-3p displays an upregulation,while TET1 displays a downregulation in human osteosarcoma cells.MiR-27a-3p inhibition could suppress the proliferation and invasion of osteosarcoma cells,contribute to cell apoptosis via negative regulation of TET1.MiR-27a-3p/TET1 might be a potential target for the treatment of osteosarcoma.