Preparation Of Calcium Phosphate/docetaxel Nanocomposite System And Its Application In Antitumor Activity

There are mainly three kinds of conventional cancer treatment methods, including surgery, chemotherapy and radiation therapy. Chemotherapy is also known as the drug treatment and occupies a very important position in the cancer treatment. The applications of chemotherapy drugs make the systemic treatments of tumor become possible and the patient survival rate was significantly improved.DTX is a semi-synthetic anticancer agent in the taxane class and it is widely used in the treatment of many solid tumors such as NSCLC, metastatic breast cancer, ovarian cancer and prostate cancer. However, the clinical application of DTX is limited by its lipophilic property.Recent years, inorganic nanoparticles as a new non-viral carriers have immense potential as a drug and biomolecule carrier system. Cap is found to be one of the most promising of them primarily because of their excellent biocompatibility, biodegradability in a physiological environment and non-toxic degradation product. Moreover, CaP has already been tested as a delivery vehicle for anticancer drugs.Base on the previous work, preparation the nanoparticles of hydrophobic DTX to improve its stability in aqueous solution and tumor targeting ability was attracted great attention in the field of drug delivery system. Here, we designed the proper method and system, based on the characteristics of DTX, to synthesis new DTX delivery system, including DTX-incorporated CaP NPs and DTX-adsorbed CaP NPs as follows:(1) CaP NPs was prepared by co-precipitation method and it was spherical and can be stability in water.(2) We prepared DTX-incorporated CaP NPs and DTX-adsorbed CaP NPs:by TEM, zeta potential, size distribution, XRD, IR and TG experiments we could confirm that DTX could be effectively incorporated into CaP NPs or adsorbed on the surface of CaP NPs.(3) The both obtained DTX-incorporated CaP NPs and DTX-adsorbed CaP NPs were spherical and well dispersion in water; the DTX-incorporated CaP NPs with a mean size around 170 nm in diameter and DTX-adsorbed CaP NPs was about 200 nm in diameter.(4) DTX-incorporated CaP NPs and DTX-adsorbed CaP NPs possessed higher anticancer activity than free DTX in vitro and DTX-adsorbed CaP NPs showed a little higher cytotoxicity than that of DTX-incorporated CaP NPs.