Fluorescent Probe Studies Of Metal Ions And Cell Imaging

Recognition of ions, molecules of fluorescent probes and imaging studies in living cells are one of the most important research subject in analysis chemistry and supramolecular chemistry. Fluorescent probes possess the advantages of high sensitivity and selectivity which have been widely used in many fields, such as biology science, medicine science, environmental testing and so on. The fluorescent prob exhibits good water solubility and membrane-permeability in living cells. Detection of metal ions in living cells have been attracted greatest interesting. The detection of ions, molecules application by cells fluorescence microscopy exhibit advantages of real-time, online, video, high sensitivity and convenient operation.I have completed the following works during this master thesis:1. The recognition ability of five coumarin fluorescent probes toward cations and anions have been investigated by titration spectra, 1H NMR, MS and ITC experiments. The stability constants and mechanism were also discussed. The five coumarin probes: coumarin aldehyde-carbohydrazide(probe 1), coumarin aldehyde-3,5-diaminobenzoic(probe 2), coumarin aldehyde-o-phenylendiamine(probe 3), coumarin aldehyde-thiosemicarbazide(probe 4) and coumarin aldehyde-ethylene diaminesalicylaldehyde(probe 5).2. All of the five coumarin derivatives exhibited a good recognition behavior with a certain metal ion. The addition of Zn2+/F-/AcO- induced an acutely fluorescence enhancement of probe 1. It was ascribed to the photoinduced electron transfer(PET) from the coumarin unit to the nitrogen atoms of imide were suppressed; probe 2 exhibits good selectivity for recognition of Mg2+ and F-, coordination of Mg2+ to the nitrogen atoms in the schiff base bond(C=N) and hydroxy moiety suppressed the PET from the coumarin moiety to the imine nitrogen atoms and the hydroxyl groups were complexed with F- by hydrogen bonds;probe 3 can be acted as a fluorescence sensor for Mg2+ by fluorescence and as a ratiometric sensor for Fe3+/F-/AcO- by UV;probe 4 has specific selectivity for Zn2+ in DMF/H2 O solvent and F- in DMF solvent; probe 5 possess fluorescence selective recognition of Mg2+/Ca2+/Fand UV absorption identification Zn2+/F- which can be used as a multiple recognition performance probe.3. Coumarin, rhodamine, quinoline, phenanthroline small molecule probe(probe 1, 4 and probe 6 to 10) and calixarene probe(probe 11, 12, 13) were used in living cells imaging studies. The probe used fluorescence enhancement or quenching, single or dual-channel, ion mobility, quantitative detection of a variety of ways to achieve detection of the metal ions include rare earth ions and anion in living cells. Probe 1 and 13 can detect metal and anion in cell imaging in addition to probe 1 can achieve intracellular Zn2+ immigration, emigration detection process; probe 6 can as a fluorescence imaging quantitative detector of Sb3+; probe 4 and 7 were detected intracellular Zn2+ and Al3+ by the blue channel; through the green channel probe 8 and 12 can response Al3+ and La3+/Lu3+ in living cells, respectively; probe 9 and 10 were detected Pb2+ and Cu2+ in living cells by the quenching of blue fluorescence, respectively; probe 11 can detect at the intracellular Ag+ level by dual-channel.These coumarin derivatives which is a kind of can not only to specific metal ions, but also exclusive response to certain anionic fluorescent and colorimetric probe, media and identification performance of each probe has its own characteristics. All of the observed results reveal that the coumarin derivatives have the potential application as chemical probes in detection metal ions and anions. Attributed to the high selectivity and sensitivity of fluorescent probes, and further combined with the fluorescence imaging technique to detect quickly relating to the life and environment of metal ions as well as some rare earth ions in living cells, which will provide a great support in the field of biology and medicine.