Preparation, Characterization And Anti-tumor Effects Of Docetaxel-targeted Nanoparticles

The aim of the present study was to characterize biotin-decorated docetaxel-loaded bovine serum albumin nanoparticles(DTX-BIO-BSANPs)and evaluate their antitumor activity in vitro and in vivo.The particle size of BIO-DTX-BSANPs prepared was found to be always lower than 200 nm,being of166.9,160.3,159.0,176.1 and 184.8 nm after redissolution with water for 0,1,2,4 and8 hours,respectively,and the zeta potential was-29.97,-26.42,-38.58,-35.14 and-25.50 m V,respectively.The polydispersity index(PDI)was stable in the range of0.159-0.178.In the in vitro drug-release study,the DTX-BIO-BSANPs targeted a human breast cancer cell line MCF-7 effectively,In vitro release experiments showed that DTX-BIO-BSANPs had a sustained release effect.In vitro cytotoxicity results showed that DTX-BIO-BSANPs inhibits proliferation of MCF-7,SGC7901,LS-174 T and A549 cells in a dose-dependent manner after exposure to DTX-BIO-BSANPs for 48 hours.An in vivo study using murine sarcoma S180-bearing mice showed that DTX-BIO-BSANPs reduced tumor growth comparable to that of docetaxel.Taken together,these results collectively indicate that DTX-BIO-BSANPs may have a potential as alternative delivery system for parenteral administration of docetaxel.