Study On The Scavenging Activity And Mechanism Of Ferulic Acid On Activated Carbonyl Compound Acrolein

Reactive carbonyl species(RCS)is a type of small molecules bearing carbonyl species.As downstream products of reactive oxygen species(ROS),RCS were formed mainly from lipid peroxidation and degradation,and can promote cells oxidative stress.Excess RCS can break the balance of redox in cells and cause cells death.Recently,there were many reports showed that RCS is in association with several chronic diseases.Acrolein,a simple and respective RCS,widely exists in human daily life.Overheat oil in food and incomplete combustion of cigarette are the two main pathways where people are exposed to acrolein.In view of toxicity and commonness of acrolein,it is necessary to look for a convenient,quickly and effective natural acrolein scavenger.Works of this paper were around this topic.The details studies are shown as follows:Scavenging activity study of ferulic acid against reactive carbonyl species acrolein:Among the 13 phenolic compounds investigated,ferulic acid was found to have the highest efficiency in scavenging acrolein under physiological condition.Ferulic acid remained 3.04%±1.89%and acrolein remained 29.51%± 4.44%after incubated with each other for 24 h.In order to clarify expression of ferulic acid in simulative cellular environment against acrolein,levels of GSH incubated with acrolein with or without ferulic acid were detected,and results supported the conclusion above.Products identification and mechanism study of reaction between ferulic acid and acrolein:Reactive products namely 2-methoxy-4-vinylphenol and 5-(4-hydroxy-3-methoxyphenyl)pent-4-enal,were identified through NMR and GC-MS,after the scavenging process.And the molecular mechanism and structure-reactivity relationship of the reaction between ferulic acid and acrolein was also studied.