| Poly(?-glutamic acid)(?-PGA)is an all natural bacterium biosynthesized polymer.?-PGA hydrogels with high water absorbability,biodegradable,biological safety and other excellent properties,which have a wide range of applications in textile environment protection,biological medical,tissue engineering and other fields.In this paper,a three-dimensional network of degradation and biocompatible compatibility protein hydrogel(?-PGA/?-PL hydrogel)was prepared by situ radical polymerization method.In addtition,? PGA/?-PL hydrogels were studied in three different valence metal ions solution(Na+?Ca2+?Cr3+)adsorption behavior.From salt bond and swelling of osmosis to explain the sweelling rule,indicating that?-PGA/?-PL hydrogels were ion-sensitive and presented more limited swelling degree in metal ion solutions with higher ionic valence number.The swelling kinetics of?-PGA/s-PL hydrogels in ions solution conforms to the pseudo-second kinetics model.SEM results shown that the NaCl adsorbed gels were full of fold,CaCl2 adsorbed gels had Clusters distributed holes on the surface and the holes distribution rules in the cross section,CrCl3 adsorbed gels were collapsed.Desorption behavior shown that?-PGA/s-PL hydrogels has a good ability of stripping,achieving the result of recycle use.Secondly,the neat structure of inorganic nanocomposite hydrogels(Ag/?-PGA hydrogels)were prepared by organic-inorganic hybrid technology.This paper studied the Ag/?-PGA hydrogel antibacterial properties,founding that nano-Ag have strong bacteriostatic bactericidal effect to gram-negative E.coli and gram-positive S.aureus bacteria.When the dosage of nano-Ag is more than 10 ug/mL,the bacteriostatic rate can reach 100%.The sustained-release kinetics of nano-Ag in PBS buffer solution was studied and found to be latent release for up to 24 h,achieving long-lasting antibacterial effect.At the same time,adding nano-Ag had no significant effect on the swelling degree and light transmittance of y-PGA hydrogels.Then,compared with medical gauze and commercial gel dressings,the antibacterial effect of nano-Ag/?-PGA hydrogel on wound healing in Balb/c mice was studied,and found that it can significantly improve wound healing,near full recovery on day 14.The use of Ag/y-PGA hydrogel can significantly reduce inflammation factor(IL-6,TNF-?)expression level and enhance the healing of wounds.Subsequently,we also studied the effect of epidermal growth factor(EGF)combined with antibacterial hydrogel to observe the recovery effect,and found that the addition of epidermal growth factor can promote wound healing more effectively.Overall,this gel was a kind of promising biological wound dressing.Finally,a new type of antibacterial cool suit was successful prepared using the?-PGA hydrogels which have superabsorbent and thermal characteristics of vaporation loss.The test result showed that the new cooling suit has obvious cooling effect,it could make the body surface temperature drop 5-7 ? to achieve the comfortable temperature for human body and effectively alleviate staff of heat stress response under the high temperature environment(40 ?).Meanwhile,also implements the stability and durability with cool suit. |
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