The Antibacterial Peptide CC31 Constitutive Body Studied The Growth, Nutrition And Rumen Development Of Lambs

The aim of this experiment is to investigat the constituted effects of two antimicrobial peptide CC31 on the growth performance,blood biochemical parameters,rumen development parameters to lambs.A single-factor 1×4 experiment was designed,and 48 7-day-old lambs are randomly assigned to four treatments,with which 3 replica were allocated in every treatment with 4 lambs.The control lamb fed basal diet,and the experimental treatments were treated with the triploid and haplotype for antimicrobial peptide CC31 300 mg/kg·DM on the basal diet,and the antibiotic control was added chlortetracycline 300 mg/kg·DM.The total experiment period is 28 days.Lambs are weighed at 14,21,28,and 35 days of age,digested trial collection of blood samples,and slaughter biochemical and chemical analysis were carried outfor each treatments.The results shows that(1)There is no significant difference in the daily feed intake among tratments(P>0.05),but the average daily gain and the feed conversion ratio of the lambs significantly increased at the 7 to 21 stage(P<0.05).The addition of antimicrobial peptides to the diets had significantly increased the liver index of lambs at the age of 14 days and 21 days(P<0.05),and have also increased the spleen index of the lambs during the whole experiment period(P<0.05).(2)The addition of antimicrobial peptides significantly improved the dry matter digestibility and crude protein digestibility of lambs at 14 and 21 days of age(P<0.05).The digestibility of crude protein in the triploid and haploid groups is respectively 10.4% and 7.8% higher than the referring group.The digestibility of dry matter in the triploid and haploid groups was higher than the control group by 4.36% and 3.76%,respectively.At 21 to 28 days of age,the addition of haploid antimicrobial peptides significantly increased the digestibility of lamb crude fat(P<0.05),which was 12.02% higher than that of the control group.(3)During the whole trial period,the dietary antibacterial peptides significantly the blood globulin levels increased at the 14 st,21th,and 28 th days of lambs(P<0.05).At 21 days of age,blood glucose levels for lamb fed antimicrobial peptides significantly increased(P<0.05).At the age of 28 days,the addition of antimicrobial peptides significantly increased the total protein content in the blood(P<0.05).(4)Diet added antimicrobial peptides significantly increased the height of the rumen-papillary tissue of the abdominal bursa of the 21-day-old lamb(P<0.05).There was no significant effect on the height of rumen papillary tissue of lambs 14,28 and 35 days old(P>0.05).(5)Adding antimicrobial peptides to diets significantly increased the content of rumen protein and propionic acid in lambs(P<0.05).At the age of 35 days,the propionic acid content in the triploid and haploid groups was higher than the control group by 13.5% and 13.9%.The triploid group and haploid group contained 56.5% and 54.3% of the bacterial protein content in the control group.The concentration of ammonia nitrogen and acetic acid in the rumen decreased significantly(P<0.05).In general,the antibacterial peptide CC31 can increase the daily weight gain of lambs,reduce the feed-to-weight ratio,promote the growth of early lambs,improve the digestibility of dry material and crude protein of the lamb,increase the content of globulin in the blood,increase ruminal bacterial protein and propionic acid content and reduce the concentration of ammonia nitrogen and acetic acid.