Inhibition Of Growth Of Mouse Liver Cancer By Intratumoral Injection Of MIP-3?

Objective:To investigate the possibility of attracting peripheral dendritic cells(DCs) to migrate to tumors and inducing specific immune response against mouse liver cancer by intratumoral injection of macrophage inflammatory protein-3a(MIP-3?).Methods:After subcutaneous liver cancer models were established, the mice were divided into3groups randomly. The mice in MIP-3? therapy group and PBS control group were injected intratumorally with MIP-3a solution or PBS respectively, while blank control group were not treated. The mice were sacrificed at day20after injection and the number of DCs, CD4+and CD8+cells in the tumor tissue were detected by immunohistochemistry. The number and phenotype of infiltrating DCs were analyzed by flow cytometry. Moreover, ten mice in each group were observed in order to draw the tumor growth curve and calculate the survival timeResults:?The number of CD4+, CD8+cells and DCs in MIP-3a therapy group were significantly higher than those in other two groups.?The expression of CD80and CD86of dendritic cells in MIP-3a therapy group was were significantly higher than those in other two groups (P<0.05)).?Tumor growth speed was significantly slower (P<0.001) and survival time was significantly longer (P<0.05) in MIP-3a therapy group than those in other two groups (P?0.05).Conclusions:?Peripheral DCs can migrate into tumor site by intratumoral injection of MIP-3?, and DCs can capture and present tumor antigen, which can effectively induce specific anti-tumor immune response.?MIP-3? may play a part in promoting maturation of DCs in the micro-environment of subcutaneous liver cancer model.