The Protective Mechanism Of Jiawei Danshen Decoction Preconditioning Induced Autophagy On Myocardial Ischemia Reperfusion Injury In Rats

ObjectTo study the mechanism of protective effect which pretreatment with Jiawei Danshen Yin(JDY) against myocardial ischemia reperfusion injury(IRI) in rats by autophagy.Methods60 healthy SD rats were randomly divided into 5 groups with 12 rats each:control(C)group, sham operation(S) group, Ischemia Reperfusion Injury(IRI)group, IRI+JDY group, IRI + JDY + Autophagy inhibitor(I)group. Model of IRI was established by ligating-relaxing the left anterior descending coronary of rats.To observe the change of Creatine Kinase(CK),Lactate Dehydrogenase(LDH);Autophagic vacuoles were observed via transmission electron microscope( TEM);The mRNA expression of autophagy related gene Beclin-1 and Atg5 were detected via Real-time quantitative reverse transcription- polymerase chain reaction(RT-PCR), and the expression of Beclin-1protein were detected by western blot(WB).ResultsThe expression levels of CK and LDH with IRI group were significantly higher than S group(P < 0.01);the expression levels of CK and LDH with IRI+JDY group were decreased significantly compared with IRI group and IRI+JDY+I group(P <0.01);TEM showed that in IRI+JDY group, autophagy of cardiomyocytes could be regulated moderately and the structure of cardiomyocytes could be improved.The expression levels of Beclin-1 and Atg5 mRNA,as well as, the expression of Beclin-1protein with IRI group were significantly higher than S group(P < 0.01);The expression levels of Beclin-1 and Atg5 mRNA,as well as, the expression of Beclin-1protein with IRI+JDY group,IRI+JDY+I group were decreased significantly compared with the IRI group(P < 0.01);The expression levels of Beclin-1 and Atg5 mRNA,as well as, the expression of Beclin-1 protein with IRI+JDY group were significantly higher than IRI+JDY+I group(P < 0.01).ConclusionPretreatment with Jiawei Danshen Yin can decrease enzyme levels of myocardial ischemia reperfusion injury in rats, improve cardiomyocytes structure, induce autophagy moderately by regulating Autophagy related genes( Beclin-1 and Atg5), which can protect the cells.