The Inhibitory Mechanism Of MiR-34a On HCMV Infection And The Effect Of Xiang A1 On HCMV Infection

Objective: 1.Discussion of mi R- 34 a has a regulation effects of HCMV, and the ways it work.2. Research on HNA-1, has inhibitory effect on whether HCMV infection.method: 1.Use the packaging lentivirus, WB, detection of viral DNA copy number,determination methods of mature virus particles to release a quantity to first determine mi R- 34 a for HCMV whether play a role of regulation.2. use online software to analyze its effect by gumming point, and use the luciferase report system and WB for validation.Later found in the experiment process of mi R- 34 a for HCMV in could have inhibition effect were analyzed, and through the detection of HCMV immediate early genes IE1,pp65(tegument) expression, then analysis the way of its inhibition to enter. 3, Chinese medicine compound to freeze-dried HNA1, drying powder, joining the cells to observe its to whether HCMV has a inhibitory effection.Results: 1. In HEL and U- 251 MG cells, overexpression of mi R- 34 a can make HCMV representative genes, DNA copy number and mature virus particle release quantity decreased significantly(P < 0.05).2. After expressing mi R- 34 a HCMV infection, found IE1 positive rate and pp65 expression was significantly decreased.In U- 251 MG cells, the cell surface receptor PDGFR alpha knock down after IE1 positive rate dropped significantly.3. Normal HEL cells after HCMV infection, join the HNA1 size can make the number of mature virus particle release was significantly decreased(P < 0.05).Conclusion: 1. In HEL cells and U- 251 MG of mi R-34 a has inhibitory effect on HCMV infection, and it is directly targeted virus genes play a role.2. Mi R- 34 a can be targeted cell surface receptor(PDGFR – alpha) suppress the virus to enter. 3.HNA-1 can inhibit the HCMV infection.