| Dietary meats play crucial role in human health. The objective of this survey was to determine the fatty acid content and composition of fresh retail beef and pork meats from 4 cities including Shanghai, Nanjing, Yinchuan and Hohhot by gas chromatography and assessed them with respect to dietary recommendations. The results showed that the total fatty acid content ranged from 4.13 g/100g (Nanjing, China) to 10.45 g/100g (Yinchuan, China) in pork and from 2.15 g/100g (Nanjing, China) to 3.48 g/100g (Yinchuan, China) in beef, respectively. A range of polyunsaturated fatty acids (PUFA) was present in all samples but the content and composition varied across samples from different regions. The total n-6 PUFA concentrations ranged from 290.54 mg/100g in beef from Nanjing to 1601.48 mg/100g in pork from Hohhot, whereas the total concentrations of n-3 PUFA ranged from 46.34 mg/100g in beef from Nanjing to 96.03 mg/100g in pork from Nanjing. The n-6/n-3 PUFA ratio was in the range from 6 to 23, which was far higher than that (5:1) recommended by WHO/FAO. Therefore, a promotion to increase n-3 PUFA dietary intake should be a concern in China.Phosphoenolpyruvate carboxykinase is a rate-limiting enzyme that catalyse glyceroneogenesis in liver and white and brown adipose tissue. The expression level of PEPCK-C in skeletal muscle is undetectable and the function is not clear. We produced PEPCK-C mice that overexpressed PEPCK-C in skeletal muscle to study the influence on exercise and enegy metabolism. Results:PEPCK-Cmus transgenic mice had more fat in muscle, got higher exercise tolerance and lower triglyceride and random blood glucose than wide type mice, but no difference on pasting glucose, weight and abdomen fat between transgenic mice and wild type mice. PEPCK-C overexpressed in skeletal muscle of PEPCK-Cmus transgenic mice improved exercise tolerance and better ability of keeping blood glucose balance, which indicated that PEPCK-C maybe important to energy metabolism. |
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