| Objective:The current study found that curcumin can inhibit tumor cell growth in vivo and vitro effectively.It can also inhibit epithelial-mesenchymal transition(EMT)in tumor cell.However,its concrete mechanism of action is unclear.This study was to investigate the effects of curcumin on SW620 cell line and to better understand its mechanism.Methods:In this study,MTS assays were employed to analyze the proliferation of curcumin-treated cells.;The Wnt signaling pathway-related genes(NKD2?Axin??-catenin and TCF4),antibodies associated with EMT(E-cadherin and Vimentin)and chemokine receptor 4(CXCR4)were examined in SW620 colorectal cancer cell lines using western blot analysis and real-time qPCR.;NKD2 small-interfering RNA(siRNA)and Wnt signaling pathway activator was synthesized and transfected into the colorectal cancer cell lines,then the Wnt signaling pathway-related genes and EMT-related genes expression levels were detected;The NKD2 and CXCR4 expression levels were detected in SW620 colorectal cancer cell lines with NKD2 small-interfering RNA(siRNA)and CXCR4 expression plasmid transfected.Results:Curcumin significantly inhibited the proliferation of colorectal cancer cells.Curcumin down-regulated the expression of ?-catenin?TCF4?Vimentin and CXCR4,up-regulated the expression of NKD2?Axin and E-cadherin in a dose-dependent manner.The Wnt signaling pathway-related genes were activated after transfection with NKD2 siRNA and Wnt signaling pathway activator.The expression of ?-catenin?TCF4?Vimentin and CXCR4 in the cells treated with curcumin was significantly up-regulated by NKD2 siRNA or CXCR4 expression plasmid transfected,and the expression levels of NKD2 and E-cadherin were down-regulated.Conclusion:Curcumin inhibits tumor epithelial-mesenchymal transition by downregulating the Wnt signaling pathway and upregulating NKD2 expression in colon cancer cells and ultimately downregulating the CXCR4 gene expression.Thus Curcumin can inhibit tumor invasion and metastasis. |
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