Protective Effect Of Sea Rabbit On Rat Liver Injury Induced By Concanavalin A

Objective: To investigate the protective effect of Aplysin on liver damage induced by the concanavalin A in rats.Methods: Forty male Wistar rats were randomly into four groups,control group and model group,low-and high-dose Aplysin treatment groups.The rats in model group,lowand high-dose Aplysin treatment groups were injected Con A at a dose of 15 mg/kg once a week for 8 weeks to establish the model of liver damage,and the rats in control group were injected with nomal saline instead of Con A.The rats in control group and model group were given the soybean oil diet and the rats in low-and high-dose Aplysin groups were treated with diet with 100 and 150 mg/(kgd)Aplysin + soybean oil once a day.The histopathological changes of liver was observed by HE staining,Masson trichrome staining and Sirius-Red staining;Olympus 400 automatic biochemical analyzer was utilized for detecting serum levels of ALT,AST and LDH;and the serum levels of TNF-α and TGF-β1 were evaluated by ELISA method.Results: HE staining revealed that disorder hepatic lobules,inflammatory cellular infiltration and interstitial lymphocytic infiltration were found in model group.In lowand high-dose Aplysin treatment groups,the situation was getting better than that in model group.The result of Masson trichrome staining indicated that interstitial collagen tissue and staining collagen was increased in model group compared with the normal control group.The result of Sirius-Red staining revealed that the amount of collagenous fiber was increased and pseudolobule was found in model group compared with control group,and in low-and high-dose Aplysin treatment groups,collagen fibrous proliferation was reduced compared with the model group.The serum levels of ALT,AST,LDH,TNF-α and TGF-β1were all increased in model group compared with the normal control group,while in low-and high-dose Aplysin groups,the serum levels of ALT,AST and TNF-α were all decreased(P<0.05),and the decrease of these indexes in high-dose Aplysin group was more obviously than that in low-dose Aplysin treatment group with statistical significance(P<0.05).and the serum levels of LDH and TGF-β1 in high-dose Aplysin groups were all decreased compared with the model group(P<0.05),while in low-dose Aplysin groups,there is unconspicuous effects.Conclusion: Aplysin could inhibit liver injury induced by concanavalin A,and its protective role might be related with the decrease of TNF-α and TGF-β1 level.