The Mechanism Of MicroRNA-125b Acting On SET Protein Inhibiting Metastasis Of Epithelial Ovarian Cancer

Background and Objective:MicroRNA-125b(miR-125b)is overexpressed in several types of cancer and contributes to chemotherapy resistance.However,its role in epithelial ovarian carcinoma remains unknown.The goal of this study was to identify the relationship between miR-125b and the epithelial-mesenchymal transition(EMT)in ovarian cancer.Method:In total,55patients with epithelial ovarian cancer(EOC)were included in our study.The relative expression of miR-125b was measured using real-time polymerase chain reaction(RT-PCR).The protein expression of SET and EMT-related indicators in cell lines were assessed by Western blot.The regulation of SET by miR-125b was confrmed using luciferase reporter assays.The effect of miR-125b on metastasis was evaluated using an in vivo metastasis model.Results:miR-125b expression was markedly lower in the EOC specimens.Ectopic expression of miR-125b in EOC cells signifcantly inhibited tumor invasion.miR-125b expression was negatively associated with both EMT and SET expression,in vivo and in vitro.Mechanistic studies identifed SET as a direct target of miR-125b,and the downregulation of SET,observed during tumor migration,was affected by the overexpression of miR125b.Conclusions:miR-125b suppresses EOC cell migration and invasion by targeting the SET protein,and this study may provide a novel mechanism for understanding the progression of EOC...