| ObjectiveTo investigate the effect of acupuncturing at Baihui(DU20)and Zusanli(ST36)acupoints on the cognitive function and the oxidation protective mechanism of cortex in vascular cognitive impairment(VCI)rats,verifying its effectiveness by the experiment,providing experimental evidence for the clinical treatment of VCI.MethodsTwo-month-old Sprague-Dawley rats were used in this study.,and the male and female were half and half,and the body mass were 200 ±20g.Twelve rats were randomly selected as the sham-operated control group.VCI rat models were prepared using the fluorescent microemboli injection through internal carotid artery.After the model was established,surviving rats were divided into three groups according to the random number table method:VCI model group(n= 12),positive drug group(n=12),electroacupuncture(EA)group(n=12).Three days after modeling,the rats in EA group were stimulated for 30 minutes with daily electroacupuncture at "Baihui" and "Zusanli"acupoints.The positive drug group was treated with donepezil hydrochloride 0.5206mg/kg orally daily for 30 days.After the model group successfully established the VCI animal model,no further treatment was performed,and the same degree of grasping stimulation was given at the same time every day.The sham-operated control group was given no more intervention and the same degree of grasping stimulation was given at the same time every day.After the treatment,the water maze test was used to test the cognitive learning ability of rats.The content of Superoxide Dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-Px)and hydrogen peroxide(H2O2)in the cortex of rats were detected by biochemical methods.Results1.In all experimental results,there was no significant difference between male and female groups(P>0.05).2.Morris Water Maze Experiment Results:①Results of Hidden Platform Test:During the 5-day experiment,the total escape distance of each group of rats was shortened day by day as the training increased.Compared with the sham operation control group,rats in the model group evaded longer total distance;compared with the model group,the total escape distance between the electroacupuncture group and the positive drug group was short,and the difference was statistically significant(P<0.05).②Results of Probe Trains:Compared with the model group,the number of passing through the platform[(7.5±1.9),(6.8±2.2)vs(3.7±1.0)]of electroacupuncture group and positive drug group was significantly higher(P<0.05),the total distance in target quadrant[(495.4±89.4)cm,(487.6±96.2)cm vs(341.4±67.3)cm]was significantly longer(P<0.05).Biochemical methods detection result:In electroacupuncture group and the positive drug group,the activity of SOD in the cortex[(17.3±3.3)U/mg,(15.1 ± 2.5)U/mg vs(9.7 ± 4.9)U/mg]was higher(P<0.05),MDA[(9.1±2.2)μmol/L,(8.4±3.7)μmol/L vs(15.2 ± 4.4)mol/L],and H2O2[(85.2 ± 16.2)μmol/L,(82.1 ± 13.2)μmol/L vs(114.7 ± 24.8)μmol/L]were significantly lower(P<0.05).In electroacupuncture,the activity of GSH-Px[(14.5±3.7)U/mg vs(9.0±2.5U/mg)was higher than that in control group(P<0.05).There was no significant difference between electroacupuncture group and positive drug(P>0.05).ConclusionsAcupuncture at Baihui and Zusanli acupoints can obviously improve the behavior scores of rats with VCI,increase the contents of total SOD and GSH-Px,decrease the content of MDA and H2O2 in brain,and enhance antioxidant effects,while inhibiting peroxidation,improve free radical metabolism. |
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