The Mechanism Of Autophagy In The Treatment Of ZDF Rats By Sleeve Gastrectomy And The Protective Effect Of EGCG On Oxidative Stress Of α Cells

Part ⅠThe mechanism of autophagy in the treatment of type 2 diabetic rats with sleeve gastrectomySleeve gastrectomy(SG)for the treatment of type 2 diabetes and loss of weight,has the advantages of simple operation,short operation time and less complications,therefore it is widely used worldwide.But the mechanism for the treatment of type 2 diabetes is not clear.In recent years,with the in-depth study of autophagy,there is constant evidence that autophagy plays an important role in glucose metabolism and lipid metabolism,which suggests the potential mechanism of autophagy for the remission of type 2 diabetes.Previous work showed that in sleeve type gastrectomy,liver fat accumulation was reversed,blood sugar decreased and insulin resistance improved in type 2 diabetic rats.The aim of this study was to investigate the changes of liver autophagy after sleeve gastrectomy in rats,and the effects in the treatment of type 2 diabetes mellitus,Part ⅡEpigallocatechin Gallate(EGCG)Prevents H202-Induced Oxidative Stress in pancreatic alpha cell line.Hypoglycemia is a major barrier to achieving stable metabolic control in patients with diabetes which is a serious clinical concern.With progression of diabetes,the ability of pancreatic a-cells which respond to hypoglycemia becomes impaired;However,it is not clear whether the dysfunctional responses of a-cells during hypoglycemia are related with oxidative stress.In the present study,we investigated whether epigallocatechin-3-gallate(EGCG)has antioxidant potential on pancreatic alpha TC1-6(aTC1-6)cell lines and protect the normal function of a-cells from H2O2 induced oxidative stress.ROS production,cell viability,glucagon secretion,and cell apoptosis were assessed.EGCG reduced ROS production and cell apoptosis,while restored cell viability and glucagon secretion within a particular concentration range.Taken together,EGCG prevented aTCl-6 cells from H2O2 induced oxidative stress,these results provide rationale for combining the conventional anti-hyperglycemia therapy and antioxidant therapy in order to avert hypoglycemia in clinical treatment of diabetes.