| Mangrove is growth in tropical and subtropical coastal and estuarine intertidal zone of the woody plant communities and created different from other ecosystem unique and rich microbial species long-term under the "limit" of the special environment frequently,which inclucding salt stress,high mineral composition,strong reducibility,acid,oligotrophic,strong ultraviolet radiatin,wind,tidal.Mangrove habitats have rich fungus species,especially the endophytic fungi which constitutes the second largest groups of Marine fung i.The interaction between different groups and environmental factors of stress,which has contributed evolution of mangrove endophytic fungi secondary metabolites synthesis pathway,unique new regulatory mechanism and related genes,the novel structure can metabolism or(and)have good biological activity of secondary metabolites,caused wide interest in natural products chemists.Over the past twenty years,a series of unique chemical structure and significant activity of mangrove endophytic fungi secondary metabolites had been reported.Mangrove endophytic fungi as a kind of Marrine microorganism resources,has become an important source of new drugs and leading compounds research and development.Ceriops tagal is a plant of Rhizophoraceae of Ceriops,and that as a medicinal plant in Hainan.In the early stage of the research,95 endophytic fungi strains were isolated from the roots,stems and leaves of mangrove Ceriops tagal,and more than ten compounds were isolated from their secondary metabolites which have novel structure and a varity of bioactive.The aim strain J5 was screened using the methods of TLC and biological activity tests with the fermentation product of the fungus in order to obtain more new antibiotic compounds.The secondary metabolites were isolated and purified by various column chromatography techniques,and their structures were identified through a combined analysis of physicochemical properties and spectroscopic evidence.The antibacterial and cytotoxic activities in vitro of the compounds were evaluated by paper disco diffus ion method and MTT method,respectively.Twenty seven compounds were isolated from marine fungus Coriolopsis sp.J5.Their structures were determined as:5-(3-methoxy-3-oxopropyl)furan-2-carboxylic acid(1),1-(5-(2-hydroxypropanoyl)furan-2-yl)pentan-3-one(2),2-hydroxy-1-[5-(1-hydroxyp e nty 1)-furan-2-yl]-propan-1-one(3),1-(5-(1,2-dihydroxypropyl)-furan-2-yl)pentan-1-one(4),5-(1-hydroxypent-4-en-1-yl)furan-2-carboxylic acid(5),5-(3-hydroxypentyl)furan-2-carboxylic acid(6),5-(1-hydroxypentyl)-furan-2-carboxylic acid(7),5-(2-carboxyvinyl)furan-2-carboxylic acid(8),methyl 5-(2-methoxycarbonylethy)furan-2-carboxylate(9),5-(2-carboxy-ethyl)-furan-2-carboxylic acid(10),furan-2-carboxylic acid(11),5-(hydroxymethyl)furan2-carboxylic acid(12),coriolospter A(13),coriolospter B(14),conocenol C(15),ceriponol E(16),(3,5-dihydroxy-6-methoxy-4-oxotetrahydro-2H-pyran-2-yl)methyl 2,3,4-trihydroxybutanoate(17),14-acetoxy-15-hydroxyirpexan(18),15a-hydroxytrametenolic acid(19),3?-hydroxylanosta-8,24-dien-21-oic acid(20),5a,8a-epidioxy-(20S,22E,24R)-ergosta-6,22-diene 3?-ol(21),3?,5a-dihydroxy-6?-methoxyergosta-7,22-diene(22),?-sitosterol(23),latifolicinin C(24),methyl-p-hydroxphenylacetate(25),hexadecanoic acid,2,3-dihydroxypropyl ester(26)and oleic acid(27),inclounding nine new conpounds and four new natural products.Bioassay results showed that compound 18 and 22 exhibited cytotoxic activity against K-562,BEL-7402 and SGC-7910 tumor cell lines.Furthermore,compound 9 and 25 showed inhibitory effect. |
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