Functional Analysis Of Seed Storage Protein Transport-Related SNARE Gene Family AtUse1

Higher plants will synthesize and accumulate a large amount of storage proteins during seed maturation.These storage proteins will be gradually decomposited into various amino acids as sources of nutrition for plant growth during seed germination and early growth.All the processes of synthesis,sorting,transport and processing of storage proteins will affect the quality and quantity of seed proteins.Plant cells have complex internal membrane systems and vesicle transport mechanism to achieve the material transport,vesicle transport including four steps:Budding,Movement.Tethering and Fusion.These steps are tightly regulated to ensure that vesicles generated from a donor compartment are delivered to their correct acceptor compartment.Many factors play important roles in the process of transport,including coat,SM proteins,cargo receptor,tethers,Rab proteins,SNAREs and so on.SNARE proteins play an essential role in membrane fusion.According to the characteristics of SNARE motif,SNARE proteins can be diveded into four groups,Qa-,Qb-,Qc-,and R-SNAREs.Each of these groups provides one of the four components of a SNARE complex,and only correct combinations of cognate SNAREs generate the functional SNARE complexes that drive specific membrane fusions.In Arabidopsis thaliana,at least 64 SNARE molecules have been identified.This number is much larger than those in other eukaryotes,suggesting a complex membrane trafficking in plants.AtUsel family,the candidate genes of Qc-SNARE in ER,may play an important role in the process of Golgi-ER retrograde transport.There is only bioinformatics forecast of AtUsel family while the function of which has not been clarified.It has great significance to figer out the function of AtUsel family in vesicle transport.