Molecular Characterization The Structural Proteins 2 Of Porcine Parvovirus Isolated In Fujian

Porcine parvovirus(PPV)is an autonomous parvovirus belonging to the genus parvovirus,subfamily Parvovirinae,family Parvoviridae.It is one of the major etiological agents of reproductive failure in pigs.Reproductive failure caused by PPV is characterized by embryonic and fetal death,mummification,stillbirth,and delayed return to estrus.In addition,PPV has been implicated as the causative agent of diarrhea,skin disease,and arthritis in swine.PPV has been reported from many different countries.PPV is composed of a linear single-stranded segment of DNA approximately 5 kb long,and its genome has more than two open reading frames(ORF).The 3' end of ORF1 encodes nonstructural proteins(NS proteins),and the 5'end of ORF2 encodes structural proteins(VP proteins).The VP proteins also can be divided for two structural proteins(VP 1 and VP2 proteins).The VP2 gene of porcine parvovirus was amplified by polymerase chain reaction(PCR)method from PPV virus isolated in Fujian Province tissues of piglets association with fetal death.Then the target fragments were cloned and sequenced.The results demonstrated that the PPV virus isolated in Fujian encodes structural proteins 2(VP2 proteins),the sequenced VP2 gene was 1740 bp in length,coding an open reading frame(ORF)with 579 amino acids.The nucleotide sequence and the amino acid sequence deduced from the gene were analyzed by the bioinformatics software.The Strongly Basic(+)Amino Acids,Strongly Acidic(-)Amino Acids,Hydrophobic Amino Acids and Polar Amino Acids were 37,48,159,213,respectively.The molecular weight,theoretical pI,total number of atoms,the instability index(?),the Aliphatic index,and the grand average of hydropathicity(GRAVY)were 64.287 Kd,5.81,8888,38.54 with classified the VP2 protein as stable,67.06,?0.568,respectively.Compared with the PPV VP2 genes published previously download from GenBank.The sequenced gene shared the highest homogeneity with JY strain(the GenBank accession number HM627652),with 86.7%,and the lowest homogeneity with IDT strain(the GenBank accession number AY684872)with 98.2%,respectively.The porcine parvovirus shared two different mainly branches in the phylogenetic tree(group I and group ?),the group I can be divided into four subgroup(?A??B??C and ?D),also the group ? can be divided into two subgroup(? E and ?F).