The Prediction Of SRNAs And Target Genes In Kineococcus Radiotolerans

Small noncoding RNAs(small RNAs,sRNAs)are a class of regulatory RNA molecules that do not encode proteins,typically a length between 50-500 nt.The sRNAs regulate the target mRNA genes by binding complementary pairing mode,thus affecting the stability and the translation repressing the gene expression.Kineococcus radiotolerans has high resistance to ionizing radiation,and it is a Gram-positive strain.The investigation of K.radiotolerans will allow a better understanding of the mechanism of radiation resistance.It will be helpful for the genetic solutions to the radiation resistance theory.sRNA is usually considered as the bacterial physiological response to the environment stress,especially in the condition of the ionizing radiation,desiccation,high temperature and high salt,sRNA inhibition may play a regulatory role,helping the organism adapt to the environment stress.The sRNA studies of Kineococcus radiotolerans are so few that this is the first investigation into the sRNAs in Kineococcus radiotolerans.The primary goal of this experiment is to identify the sRNAs in Kineococcus radiotolerans.Secondly,the potential sRNAs that may be involved in the radiation resistance will be predicted for their target genes.Another important goal of this experiment is based on the sRNAs and their target genes prediction,combined with computer programming technology to develop an interface of visual integration platform with the bioinformatics software for the subsequent lab experiments providing a pipeline platform.The sRNAs in Kineococcus radiotolerans are predicted by this platform with 53 sRNA candidates screened.There are 217 sRNA candidates screened by the transcriptome sequencing analysis.Two sRNA candidates were predicted having the possibility to interact with the differentially expressed genes by the sRNA target prediction.