Inactivation And Mechanism Of Vibrio Parahaemolyticus By Combination Of LED And Botanical Bactericide

Vibrio parahaemolyticus,a kind of gram negative bacterium.It�s widely distributed which presented in coastal waters.The food poisoning caused by Vibrio parahaemolyticus is the most important reason in our country,and it's the primary cause of coastal areas bacterial food poisoning,which is harmful to the human body and cause great loss of property.All along,Vibrio parahaemolyticus commonly used sterilization technology is basically in the traditional industrial sterilization method,such as heat treatment,UV sterilization,ultra-high pressure and other industrial sterilization methods to reduce the number of Vibrio parahaemolyticus in food.These methods are often limited by the cost and the equipment,and will have a negative impact on the sensory and quality of the food.Therefore,it's significantly to explore the methods which are effective,safe and not lead to the drug resistance or change the quality of food to control microbial infection such as Vibrio parahaemolyticus.This paper intends to explore the inhibitory effect of new antibacterial method of synergistic effect of LED and plant-based bacteriostat on Vibrio parahaemolyticus in food model under low temperature.On the basis of creating a new LED sterilization device,the effect of sterilization not only in PBS but also in fresh shrimp was studied.And the antibacterial mechanism of the synergistic effect of LED and plant-borne bacteriostat was studyed either.The results are as follows.(1)The LED sterilization device with the power of 20 W,the wavelength of 405 nm,in collaboration with natural antibacterial agent citral,has a significant inhibitory effect in Vibrio parahaemolyticus.Control the bacterial suspension in PBS buffer for 5 minutes or 120 minutes for the shrimp food model soaked in Vibrio parahaemolyticus.The number of Vibrio parahaemolyticus could be reduced to below the detection limit and has little effect on the sensory quality of fresh shrimp food and is more efficient than the bactericidal effect of LED or citral alone at the same time,Thus,the synergistic effect is more efficient,more quickly inactivated bacteria than the LED or citral at the same time.(2)The bactericidal treatment of Vibrio parahaemolyticus was carried out by three different methods: LED group,citral group,LED and citral group.The initial bactericidal mechanism was studied from the cell morphology of Vibrio parahaemolyticus,the integrity of cell membrane permeability,and cell fat oxidation.The study will provide a new method for the effective control of Vibrio parahaemolyticus in seafood.It is more effective,safer,and less resistant to drug resistance and not change the sensory traits of food,which provides a scientific basis for the industrial application of low temperature new sterilization technology for LED synergistic fungicide.