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Characteristics Of Bacteria And Denitrifying Microorganism Distribution In The Different Vegetation Area Along The Riparion Zone Of Erhai Lake

Posted on:2018-04-14Degree:MasterType:Thesis
Country:ChinaCandidate:X Y ZhaoFull Text:PDF
GTID:2370330533468476Subject:Environmental Engineering
Abstract/Summary:PDF Full Text Request
The riparian zone is the important protection barrier of lakes,and the microorganisms of different vegetational area in the riparian zone play the key role for the influent pollutants degradation.Shapingwan riparian and Zhouchengwan riparian zones of Erhai Lake were selected as the object to study the characteristics of bacteria and denitrifying microorganism distribution of the different vegetational areas,which were the arbor grass vegetation area of Shangpingwan and Zouchengwan(hereinafter referred to as SQ and ZQ),emergent plant area of Shangpingwan and Zouchengwan(hereinafter referred to as ST and ZT),submerged plant area of Shangpingwan and Zouchengwan(hereinafter referred to as SC and ZC)and unvegetated area of Shangpingwan and Zouchengwan(hereinafter referred to as SW and ZW),using Illumina HiSeq high throughput sequencing and QPCR(Quantitative polymerase chain reaction)molecular approach.The research results were illustrated as follows.(1)The bacteria species were very abundant of different vegetational areas in Shangpingwan and Zouchengwan riparian zones of Erhai Lake,and there were 54 phylum,106 class,192 order,334 family and 503 genus.The predominant bacteria community were Proteobacteria,Firmicutes,Chloroflexi and Acidobacteria.(2)The diversity of bacteria had a significant difference in the vegetational areas of Zhouchengwan and Shapingwan riparian zones of Erhai Lake.The bacteria OTUs number sequences of vegetational areas in Shapingwan riparian zone from high to low were as follows: ST(3162),SQ(2926),SW(2709)and SW(2427),and the diversity Shannon indices sequences from high to low were ST(10.14),SQ(9.75),SC(9.44)and SW(9.26).The bacteria OTUs number sequences of vegetational areas in Zhouchengwan riparian zone were as follows: ZW(2816),ZT(2655),ZC(2451)and ZQ(2047),and the diversity Shannon indices sequencefrom high to low were ZT(9.61),ZQ(9.06),ZW(8.96)and ZC(8.70).In general,the bacteria diversity of Shapingwan riparian zone was much higher than Zhouchengwan riparian zone.(3)The canonical correlation analysis indicated that TP,pH and OM were the most important environmental factors which affected the bacterial community in Shangpingwan and Zhouchengwan riparian zone of Erhai Lake.(4)Quantitative analysis showed that the abundance of AOA is higher than AOB in the surface soil and sediment of Shangpingwan and Zhouchengwan riparian zone of Erhai Lake.The abundance of AOA and AOB decreased significantly with the depth of soil and sediment,and AOA is higher than AOB in most sample points in Shangpingwan riparian zone.The number of advantages increases the possibility that AOA would be the main driver of ammonia oxidation in Shangpingwan and Zhouchengwan riparian zone of Erhai Lake.(5)Quantitative analysis showed that the abundance of nirK was higher than nirS in the surface soil and sediment of Shangpingwan and Zhouchengwan riparian zone of Erhai Lake.The abundance of nirK and nirS decreased significantly with the depth of soil and sediment,and nirK was higher than nirS in all sample points in Shangpingwan riparian zone.The number of advantages increases the possibility that the bacteria of nirK would be the main driver of denitrification in Shangpingwan and Zhouchengwan riparian zone of Erhai Lake.(6)Statistical analysis showed that OM promoted the growth of AOA and denitrifying microorganisms,but had no significant effect on AOB.At the same time,the abundance of AOB was controlled by ammonia nitrogen.,and there was significant positive correlation relationship between pH and nirS.
Keywords/Search Tags:high throughput sequencing, QPCR, the riparian zone of Erhai Lake, bacterial community, denitrifying bacteria
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