A Preliminary Study And Application Of An Arsenic-Oxidizing Bacteria

Soil and water arsenic pollution has become an important environmental problem.In this study a high arsenic resistant bacteria was isolated from an arsenic-containing industrial wastewater sample collected from Daye Non-ferrous Metals Company,China and named as P11.And the strain was identified as Pseudomonas.Strain P11 can grow at a wide range of temperatures from 4°C to 37°C;the optimum is 30°C.It can proliferate in a pH range of 7.0-9.0;the optimum is 7.0.This strain possesses the ability to resist several heavy metals and efficiently precipitate arsenic.Here,we described the high quality draft genome sequence,annotations and the features of P11.The genomic sequence is 6,644,817 bp with a G+C content of 62.20% and contains 6,143 protein-coding genes,250 pseudo genes and 76 tRNAs/rRNAs genes.Many gene clusters responsible for tolerance or detoxification of metal cations were identified in the genome.The genome sequence provides important insights to illuminate the detoxification mechanisms of Pseudomonas species that inhabit heavy metal-contaminated environment.In the application of this strain,immobilized microorganisms(IMO)was adopted in this study.The optimum ratio of embedding materials was obtained by orthogonal experiment: 1.5% PVA,0.625% alginate,0.5% diatomite.Compared with the free cells,arsenic removal has obvious effect,which provides a new idea for the treatment of arsenic containing wastewater.