Isolation Of Uranium Resistant Bacteria In Uranium Tailing And Analysis Of Functional Proteins

The development and utilization of uranium mines have produced radioactive contaminants that are both radioactive and chemically toxic and pose potential threats to the environment,ecology and the human body.Due to its advantages of high efficiency and low cost,microbial technology has become a research hotspot in the field of uranium-containing wastewater purification.In this study,indigenous uranium-tolerant bacteria were isolated from the soil of a uranium tailings area in southern China,purified and isolated by bacteria,and identified as indigenous species.The total proteins and phosphatase activities of the indigenous bacteria were measured under optimal environmental conditions.The total protein content and phosphatase activity were measured using scanning electron microscopy?SEM?,X-ray energy spectroscopy?EDS?,Fourier transform infrared spectroscopy?FTIR?and X-ray.XRD analysis of indigenous uranium removal mechanism.The test results are as follows:5 strains was isolated from soil of uranium tailings uranium resistant bacteria,respectively named the A,B,C,D and E,under the condition of 30?,pH=7.0,within 24 h of removal of low concentration of uranium are above70%,including A and B of different initial concentration of uranium?VI?removal rate more than 90%in the inner world of 8 h.Physiological and biochemical tests found that all five bacteria were aerobic bacteria,of which A had an optimal growth temperature of 30? and an optimum pH of 6,and it could grow well in glucose and sodium lactate.B optimum growth temperature was 30?.The pH is 7 and it can grow well in glucose;the optimal growth temperature of C,D and E is 30-37? and the optimum pH is 7-8,can grow well in glucose.Selection in addition to the better effect of uranium A?klebsiella?and B?acinetobacter?protein test,it is found that the total protein content of klebsiella from 54.50?g/mL before eliminating uranium into in uranium after 42.81?g/mL,phosphatase activity by 18.59U/L before eliminating uranium increased to 27.716U/L after eliminating uranium,FTIR,SEM,EDS and XRD analysis showed that bacteria of carboxyl,hydroxyl,phosphate groups and amino functional groups to participate in the removal of uranium,are attached to the surface of bacteria,and the precipitation of uranium deposits contain uranyl phosphine hydrate[UO₂?H₂PO₂?₂·H₂O]and uranyl phosphate hydrate[?UO₂?₃?PO₄?₂·4H₂O];The total protein content of acinetobacter from 50.26?g/mL before eliminating uranium into 40.01?g/mL,after eliminating uranium phosphatase activity by 18.59U/L before eliminating uranium increased to27.72U/L after eliminating uranium,FTIR,SEM,EDS and XRD analysis showed that protein amide,hydroxyl and carboxyl functional groups and phosphate groups should participate in the reaction of uranium,uranium were adhered on the surface of the bacteria in the sediment,and the precipitation containing uranyl phosphate hydrate[-?UO₂?₂?PO₄?₂·4H₂O]and uranyl phosphate[UO₂?PO₃?₂].