Nano Metal-organic Frameworks Based On Porphyrin Derivatives In Biomedicine Application

Nano Metal-Organic Frameworks?NMOFs?are a kind of Novel Crystalline Porous Nanomaterial.It has a wide range of applications in biomedical sciences such as fluorescence imaging,drug delivery,photodynamic therapy?PDT?,photothermal therapy?PTT?.The theoretical coordination number of Zr in the porous zirconium-based MOF UiO-66 is 8,and there are 12 terephthalic acid ligands around the Zr cluster,but there will be ligand defects in the actual structure.Therefore,during the experiment,this phenomenon can be used to adjust the defect level by adding different kinds of ligands.In this paper,we report a defected UiO-66 type Nanoparticles?ZnDiCPp-I₂?UiO-66?by using a one-pot.Cellular experiments show that ZnDiCPp-I₂?UiO-66 has excellent photodynamic therapy capabilities;The nanocomposite Py-UiO-66/C was prepared by a one-pot method.Cell experiments show that the composite material has certain photothermal and photodynamic therapy effects,and the combination of photothermal and photodynamic therapy results in a significant enhancement of the therapeutic effect.?.Using a coordination defect design strategy,a one-pot method was used to dope ZnDiCPp-I₂?UiO-66 with the heavy atom modified porphyrin photosensitizer ZnDiCPp-I₂doped into UiO-66 NMOF.The resulting material has good chemical stability and light stability in a tissue simulation environment.Singlet oxygen generation experiments show that ZnDiCPp-I₂?UiO-66 has satisfactory singlet oxygen generation ability when UiO-66 is doped with very low content of ZnDiCPp-I₂,which is attributed to the remarkable iodine atom heavy atom effect.Intracellular experiments show that ZnDiCPp-I₂?UiO-66 has ignorable dark toxicity and good biocompatibility to Hep G2 cancer cells;Zn DiCPp-I₂?UiO-66 has excellent photodynamic therapy capabilities.Compared with similar reported MOFs photosensitizers,ZnDiCPp-I₂?UiO-66 has the lowest effective photosensitizer concentration under low-power LED illumination.A promising photodynamic treatment platform.?.Using the dehydration polymerization of glucose under solvothermal conditions,nanocomposites of porous carbon and UiO-66-NH₂ were prepared by one-pot method.With the strong absorption capacity of porous carbon in the visible region,the composite exhibits a certain photothermal performance.Further use of UiO-66-NH₂ self-coordinating defects,the introduction of porphyrin?PyCOOH?photosensitizer to obtain Py-UiO-66/C composites.PyCOOH is a common photosensitizer that imparts photodynamic properties to the composite.Both the photothermal and photodynamic properties of the composites are wavelength selective.When irradiated with red light at 660 nm,the composites have photothermal properties without photodynamic properties.When irradiated with green light at 540 nm,the composite has photodynamic properties without photothermal properties.Cell experiments show that the composite material has certain photothermal and photodynamic therapy effects,and the combination of photothermal and photodynamic therapy results in a significant enhancement of the therapeutic effect.