Study On The Physiological Characteristics Of 11 Strains Of Bacteria In The Rumen And Optimization Of Fermentation Conditions

Rumen microorganism,a microbial population in the rumen,can rapidly degrade the natural fibrous substance in the food and turn into a family of animal nutrition.In this paper,a series of studies on 11 strains in the laboratory were discussed.1.The purpose is to find strain with the high activity of cellulase,strong tolerance and anti-bacterial.The activity of cellulose enzyme was determined by the method of 3,5-dinitrosalicylic acid(DNS method),the study of tolerance was applied to the coating plate counting method,and the anti-bacterial was studied by the Oxford cup method.The results showed that the cellulase activity of the strains T-2,T-3 and T-10 was higher.The strains T-2,T-5,T-7,T-8 and T-9 showed strong acid resistance.The strain T-4,T-5,T-9 and T-11 showed strong resistance to bile salts.The strain T-4 and T-7 had a strong anti-bacterial effect on the vibrio parahemolyticus.T-7 showed a strong anti-bacterial activity to the Aerogenes.T-5,T-9and T-10 showed a strong anti-bacterial activity to Escherichia coli.T-3 had a strong anti-bacterial activity to the Sarcina lutea.2.To improve the activity of cellulase activity and antimicrobial activity,the culture medium of the selected excellent strains was optimized.The results showed that for fermentation medium of cellulase production the best carbon source is glucose,and the best nitrogen source is peptone.For the fermentation medium that inhibits pathogenic bacteria,the best carbon source is glucose,and the best nitrogen source is yeast extract.Moreover,the compatibility of 11 strains was studied to determine whether there was an antagonistic effect among the 11 strains,so as to determine the strain that could be mixed and combined.With cellulase activity determination and other experimental results,the optimal complex combination of cellulose and the possible optimal combination of anti-bacterial was determined.In these complex combinations,there were two combinations of bacteria and three strains of bacteria,and the strains in the two strains were tested in 1:1,1:3 and 3:1,and the strains in the three strains were tested at 1:1:1,and finally the optimal combination ofcellulose and anti-bacterial optimal combination was determined.The experimental results showed that the strain T-2 and T-3 were the optimal combination of cellulose with a 1:1 ratio,and the strain T-4 and T-5 were combined with a 1:1 ratio to be the optimal combination of the inhibitory bacteria.3.To further improved the activity of cellulase,fermentation conditions for the optimal combination of cellulase were optimized.The experimental results showed that the optimum fermentation conditions for cellulase production are initial pH of 6.5,quantity of 5%,fluid volume of 25% and fermentation temperature of 37 ?.4.The effect of pH and other conditions on the anti-bacterial active substance was studied,to determine whether the anti-bacterial activity produced by bacillus is fully functional in the rumen.The experimental results showed that the antimicrobial active substances at pH 6.5,dilution ratio is 2.0,the temperature of 37 ? condition have a strong anti-bacterial activity.Therefore,in the rumen environment with a pH of 5.5-7.5 and a temperature of 38 ?,anti-bacterial active substances can play a anti-bacterial effect.