Study On The Safety Evaluation And Imaging Distribution Of Carbon Nanomaterials In Cells

In recent years,new nanomaterials have received great attention due to their widespread use in agriculture,food safety and pharmacy.Among them,graphene oxide(GO)and carbon quantum dots(CDs),as a new generation of nanometer-scale materials,which may have a variety of strange characteristics,and have become the most popular scientific research hotspot in the world.The example of the encapsulation of living Hela cells with GO(Hela@GO)is reported.Here the cell viability,reactive oxygen species(ROS),membrane integrity and apoptosis were compared between Hela@GO and control Hela cells by being exposed to four kinds of organic solvent,including dimethyl sulphoxide(DMSO),ethanol,acetone,and glycerin.It was demonstrated that the GO can protect Hela cells against the stress induced from organic solvent by keeping the membrane integrity,decreasing the ROS level and restraining the apoptosis.In addition,carbon nanomaterials are also widely used in the field of cell imaging.MSCs hold great promise as cell therapy candidate in clinics.However,the underlying mechanisms remain elusive due to the lack of effective cell tracking approaches during therapeutic processes.CDs have been found a wide range of biological applications as fluorophores due to their extraordinary brightness and high photostability that are far superior to those of conventional organic dyes.These traits are particularly appealing for studying cell biology under a autofluorescence background of cells in a long-term observation period.However,the intracellular distribution of CDs in the cell is not clear.In this syudy,CDots were used to label MSCs and induced them to osteogenesis to observe the long-term imaging effect of carbon dots.In addition,we used the organelle-CDs co-localization method to explore the specific location of carbon dots in the MSCs.