Investigation On Marmota Caudata Virome,Discovery And Characterization Of Novel Viruses In Xinjiang

In recent years,new infectious diseases are common,more and more new infectious diseases,more and more complex,and more severe prevention and control situation,have become a serious threat to human health and social development.Therefore,the effective prediction and prevention and control of new onset infectious diseases has become one of the major scientific problems to be solved in human public health.There are about 2277 species in the world,accounting for about 42% of all mammals.Rodents are the most widely distributed and most diverse mammalian species in the world.They are also one of the most important natural hosts of zoonosis.They can carry more than 180 viruses.Therefore,it is of great significance to understand the system of rodent virus in the early warning system of new infectious diseases.At present,there is a lack of research on rodent carrying virus group at home and abroad.In our country,there is a lack of large scale research on rodent carrying virus group.Marmota caudata,as a special species in the Pamirs Plateau,has not been carried out in the study of its viromes,and the Powassan Virus(POW),Rabies virus and Woodchuck Hepatitis Virus(including Hepatitis B virus,Hepatitis D virus)that cause encephalitis are found in the North American marmot,so it is necessary to study the marmot carrying viromes.Objective: To understand the background data and potential new virus of the virus carried by Marmota caudata in the Pamirs Plateau in order to provide basic data for predicting and preventing the new onset of zoonosis in the source of marmot.Methods: The anatomy of marmots,lungs,intestines,liver,kidney,lymph and spleen preservation reserve.The samples of the lungs,intestines,kidneys and lymphoid tissues were mixed to extract the virus nucleic acid in the mixed sample,and reverse transcriptional c DNA and add anchored primers.Then the dsc DNA was synthesized and the 5 μL electrophoresis was taken by PCR.The qualified samples were sent to the Huada Company of Shenzhen for high-throughput sequencing.The sequencing results were analyzed by virus metagenomics and the virus prevalence was detected,full gene amplification,genetic evolution analysis,and so on,according to the results of macrogenomics.Results:(1)Collected a total of 101 Marmota caudata,the virus through metagenomic analysis,found a total of 25 families,including vertebrate virus 13 families and 18 genera,the insect virus has 2 families and 2 genera,plant virus has 6 families and 6 genera and virophage has 1 family and phage has 3 families and 25 genera.This project focuses on the identification and analysis of Adenoviridae,Herpesviridae,Astoviridae,Anelloviridae,Circoviridae,Polyomaviridae,Parvoviridae,Reoviridae,Picornabiviridae and Picornaviridae.The positive rate was found to be 1.0%-76.2%.A preliminary genetic analysis of the detected fragments was carried out.It was found that the detection fragments of the adenovirus were divided into two groups,and three segments of DNA Pol,Hexon and IVa2 were obtained.The herpesvirus detection fragment was divided into two groups,and a DNA Pol gene was obtained;the detection fragment of the astrovirus could also be divided into four groups;the detection fragment of the anellovirus was three diseases.The detection fragment of parvovirus was two virus genes,the detection fragment of the picobirnavirus was the same virus gene,and thepicornavirus detection fragment was four virus genes.(3)The complete genome sequences of two strains of circovirus and three strains of polyomavirus were obtained.Conclusion: This study conducted a comprehensive survey of the Xinjiang Marmota caudata virome by the method of viral metagenomics.The epidemiological investigation of 11 vertebrate virus families,1 insect virus family,and 1 Lavidaviridae in Xinjiang Marmota caudata were found and verified.According to the latest ICTV classification criteria,preliminary genetic evolution analysis shows that all are new virus species or subspecies.Among them,two new strains of circovirus and five new adenovirus,a polyomavirus,a herpesvirus and four astrovirus,imply that it may be a new species.The study of Marmota caudata in Xinjiang was studied by virus metagenomics.The discovery of these viruses enriched the data of rodent viromes,and reflected the breakthrough effect of virus metagenomics analysis on the efficiency of virus information mining.