The Mitochondrial Genomes Of Thirteen Bombycoid Species And Theirs Phylogenetic Implications

Bombycidae sensu lato,belonging to Bombycoidea,is one of the important familes in Lepidoptera,which including the well-known silk resource insect and the pests of agriculture and forestry.The mitochondrial genomes(mitogenomes)of 13 bombycoid and 1lasiocampid species have been used to clarify the genome characteristics,and compared with the other sequenced 19 bombycoid mitogenomes.Furthermore,the phylogeny of Bombycidae s.lat.has been reconstructed based on these data.The main 3 results were followed:1.Thirteen bombycoid mitogenomes were both closed-circular double strands DNA molecule which contained 37 genes and a putative A+T rich region.There are some special characteristics in our study's mitogenomes,a long non-coding region had been found between trnA and trnR in Mustilia undulosa in which existing some microsatellite-like region;the stop codon of the cox1 of Ganisa cyanogrisea is complete;T.daii could fold into the typical cloverleaf structure with the complete the dihydrouridine(DHU)arm;and so on.2.The length of the completely sequenced bombycoid species in our study were ranged from 15,355 bp to 15,880 bp,and these bombycoid mitogenomes illustrated significant AT bias.In protein coding genes,the most frequent 4 codons were UUA(L),AUU(I),UUU(F)and AUA(M),respectively.3.The phylogenetic implications of 13 bombycoid species were reconstructed based on the mitogenome was showed that the two bombycid subfamilies,Oberthueriinae and Prismostictinae,were combined as one clade supported by bootstrap value of nearly 100 and posterior probability of 1.And the phylogenetic relationships within Bombycoidea were:((((Bombycinae + Sphingidae)+ Saturniidae)+(Oberthueriinae + Prismostictinae))+Eupterotidae)and determined that a conclusion that Bombycidae s.lat.was a polyphyletic group.