Study On The Metabolic Network Of Ion-beam-recombinant DOB Based On Bacterial Genome De Novo Sequencing

In order to further understand the biological effect of low energy ion implantation on the physiological metabolism of DOB,based on the DOB whole genome De novo sequencing data,the genomic structure and function of the ion beam recombinant bacteria DOB981,DOB113,DOB073 and the original strain DOB150 were annotated by bioinformatics,and the gene scale was constructed.The metabolic network was visualized by Cytoscape.The RAST annotation showed that the genome of the recombinant strain DOB073 increased by 12883 BP and 49 more than that of the original strain;the genome of the recombinant strain DOB113 increased by 11174 BP and 44 more than that of the original strain;the genome of the recombinant strain DOB981 decreased by 223268 BP than the original strain,and the ORF decreased by204.The results of metabolic network analysis showed that the number of reaction substrates and biochemical reactions in the recombinant DOB113 and DOB073 metabolic networks were the same,while the DOB981 reaction substrate of the ion beam recombinant bacteria was three more than that of the original strain,and the number of metabolic reactions decreased by 10.The Cytoscape visualization analysis showed that the DOB981 metabolic network contained 1604 nodes and 3733 connections,although 1 nodes were increased compared with the original strain,but the line was reduced by 68.The results of topological attribute analysis of genetic scale metabolic network showed that the metabolic networks of DOB981,DOB113,DOB073 and the original strains were all scale-free networks,and had small world network(SWN)characteristics,but the characteristic path length of the metabolic network of recombinant bacteria DOB981 was larger than that of the original strain,and its overall structure was relatively loose.Metabolic pathway analysis showed that all metabolism of DOB in the whole life body was contained in 73 metabolic pathways of KEGG,in which the recombinant bacteria DOB981 increased 2 carbonmetabolizing enzymes and 1 carbon metabolism and 4 nitrogen metabolizing enzymes were missing from the original strain,thereby further improving the adaptability of the bacteria to the oligotrophic environment.This study not only has important significance for the research of DOB's environmental adaptability mechanism,but also provides a theoretical basis for the construction of DOB genome metabolic network.