Application Research On The Effect Of ?-ray On Biology

Gamma rays are high-energy electromagnetic wave,and their strong effects on biological macromolecules result in genetic and physiological effects on living things.Different biological samples were treated with gamma rays irradiation,and 25 Gy was used to treat the graine with particles,and the effect of radiation detoxification was observed.With 1000 ~7000 gy of absorption strength of 60 co-gamma rays,we treat some biological species as follow.1.The treatment of pine wood nematode can obtain the dose death curve of pine wood nematode.2.The treatment of bacteria and yeast can observe the survival rate of cells after treatment,3.The treatment of filamentous fungi can observe the effects of irradiation on mycelial growth and sporulation of fungi,4,The treatment of plant seeds can observe the effect of irradiation on plant seed germination and young shoot growth.,The following results can be obtained:(1)Irradiation and the Nosema bombycis illness will reduce graine hatching.After irradiation treatment of poisoned graine,hatching rate decreased more than that of non-toxic graine.(2)The incubation time of the healthy graine is earlier than that of graine infected with the N.bombycis illness(1 ~ 2 d),and irradiation treatment can further delay the incubation time,resulting in a decrease in the infection rate of N.bombycis and the virus index of the hatching ant worms in the irradiation group.(3)The total graine infected with the N.bombycis illness increased after irradiation treatment.It may be that when the number of micro-particles in the graine is very small,there will be no result of detecting N.bombycis,and irradiation may reduce the ability of graine to inhibit N.bombycis and promote the proliferation of small particles in graine.Irradiated unhatched graine have a lower infection rate of N.bombycis than non-irradiated graine.This may be due to a higher proportion of non-toxic graine derived from hatched larvae,resulting in unirradiated unhatched virulent silkworms.Increased infection degree of N.bombycis,and the hatching rate of the irradiated graine is very low,so that the pre-hatching and post-hatching graine are basically unchanged.(4)Low infection rate of N.bombycis(62.5%)of graine,with the increase of radiation dose rate,the detoxification effect can reach more than 90%.However,the high infectionrate of N.bombycis(>70%)of graine still had greater than 70% detoxification effect after irradiation.The detoxification mechanism was the low hatching rate of the graine after irradiation.(5)The total amount of cerium,the weight of cerium and the amount of cerium layer all decreased after irradiation,and increased with the increase of dose rate.The helium layer rate increased with the decrease of dose rate(r = 0.84).However,the experimental sample is smaller,and the effect of irradiation on the yield of silkworm cocoon and the promotion of silkworm cocoon production need to be further repeated.(6)Bursaphelenchus xylophilus can tolerate radiation under 7000 Gy,and pine wood nematode can survive and reproduce.With the increase of radiation dose,the instant survival rate decreases.With the increase in the number of culture days and irradiation dose,the survival rate showed a gradual decline,while in the third generation of resuscitation cultivation,the number of pine wood nematodes decreased significantly.(7)The radiation dose of 1000 Gy can completely kill four tested bacteria.(8)Saccharomyces cerevisiae and Rhodotorula spp.are resistant to irradiation,and filamentous fungi have different susceptibility to irradiation.Irradiation inhibits penicillium spp.colony expansion,inhibits Sporulation of Aspergillus niger and has no effect on Mycelium expansion and Rhizopus Irradiation(9)The tolerance of plants to radiation is very different.Brassica oleifera is resistant to radiation,irradiation basically does not affect its germination rate and individual growth.Lettuce is the most sensitive and the germination rate drops when ?4000 Gy.Irradiation does not affect the germination time of millet and leeks,but it decreases the germination rate linearly with the increase of irradiation dose.The germinated individual has a small body,and high-dose(millet ?4000Gy,leeks ?2000Gy)buds die.Irradiation did not affect the germination time of wheat seeds.High-dose(?4000 Gy)irradiation had an effect on the germination rate of wheat seeds.The germination of individuals treated with each dose had little growth,and high-dose(?5000 Gy)young shoots died.