Study On The Formation Of Biofilm On The Surface Of Fish Scale By Vibrio Parahaemolyticus And Escherichia Coli

Food-borne pathogens can be adsorbed not only on non-biological materials such as glass,stainless steel,and plastics that are commonly used in food processing and food transportation,it can also form biofilm on adsorption surface which was hard-to-remove,resulting in cross-contamination,which will cause serious public health hazards.At present,researches on biofilms are mainly concentrated on the surface of non-biological materials.Therefore,it is very important to study biofilm biofilms.In this paper,Vibrio parahaemolyticus and Escherichia coli in foodborne pathogens were studied.First,the dynamic process of the biofilm formation on the surface of fish scales at the optimum temperature(37 oC)was analyzed by electron scanning microscopy and extracellular polymer analysis,and the formation of biofilms on the surface of fish scales at different temperatures(4,10,15,25,37 and40 oC)under different temperature conditions was studied.Secondly,the eradication of acidic electrolyzed water and sterile deionized water on the biofilm formation on the surface of fish scales were studied.Finally,the extracellular polymer analysis and atomic force microscopy were used to analyze the difference in the adhesion ability of Vibrio parahaemolyticus and Escherichia coli in biofilm formed on fish scale.The dynamic process of biofilm formation on the surface of fish scales by Vibrio parahaemolyticus and Escherichia coli was analyzed using an electronic scanning microscope technique combined with an extracellular polymer analysis method.The results showed that Vibrio parahaemolyticus and Escherichia coli have significant time differences in the formation of biofilms,but all experienced four stages of bacterial formation of biofilms:the adhesion period(0-24h)and(0-36h),biofilm formation period(24-36h)and(36-48h),biofilm maturity period(36-48h)and(48-60h),dissemination period(after 48h)and(after 60h);The ability of Vibrio parahaemolyticus and E.coli to form a biofilm on the surface of fish scales at different temperatures:37 ~oC>40 ~oC>25 ~oC>15 ~oC>10 ~oC>4 ~oC?The effect of acidic electrolyzed water and sterile deionized water on the removal of biofilms formed on the surface of fish scales by Vibrio parahaemolyticus and Escherichia coli was studied using an electronic scanning microscope technique combined with an extracellular polymer analysis method.The results showed that the acidic electrolyzed water had the scavenging effect on the biofilms formed on the surface of fish scales under all temperature conditions,and the scavenging ability of E.coli biofilms was higher than that of the biofilms of Vibrio parahaemolyticus.After soaking for 5 min,the biofilms of both bacteria became thin,the three-dimensional network structure disappeared,and the bacteria appeared rough surface or even appeared to be broken.Prolonged treatment time The removal efficiency of acidic electrolyzed water on biofilms also increased significantly.After 10 min of treatment,the clearance rates of acidic electrolyzed water against Vibrio parahaemolyticus and E.coli biofilms reached over 60%and 90%,respectively.The differences in the adhesion of biofilms formed on the surfaces of fish scales and glass surfaces by Vibrio parahaemolyticus and Escherichia coli were studied preliminarily using extracellular polymer analysis and atomic force microscopy.The results showed that the adhesion ability on the surface of fish scales was:Vibrio parahaemolyticus biofilm>E.coli biofilm;Adhesion ability on glass surface:Vibrio parahaemolyticus biofilm>E.coli biofilm;Vibrio parahaemolyticus on scale surface Biofilms and E.coli biofilms adhere more strongly than they do on glass.